摘要
目的:探讨激肽家族成员C1(KIFC1)在胆管癌中的表达与作用及其调控机制。方法:通过GEPIA数据库探究胆管癌组织和正常组织中KIFC1的表达,采用实时定量PCR方法验证。实验选择人RBE和9810细胞,采用细胞计数试剂盒-8法检测细胞活力,采用Transwell检测细胞迁移和侵袭能力。采用FITC/PI法检测细胞凋亡。用蛋白质印迹法分析蛋白表达的变化。结果:KIFC1在胆管癌组织中的表达明显高于非肿瘤组织。KIFC1高表达可能与胆管癌预后不良有关。抑制KIFC1可能通过影响PI3K/AKT通路降低胆管癌细胞的增殖、迁移和转移能力,并促进细胞凋亡。结论:KIFC1可促进胆管癌的恶性生物学行为进展,其机制可能与KIFC1调控PI3K/AKT通路相关。KIFC1可能成为指导胆管癌诊断和治疗的潜在靶点。
Objective:To investigate the expressions and role of kinesin family member C1(KIFC1)in cholangiocarcinoma and its regulatory mechanism.Methods:The expression of KIFC1 in cholangiocarcinoma and normal tissues were investigated by GEPIA database.Real-time quantitative PCR(qRT-PCR)was used to verify the results.Experiments were carried out with human RBE and 9810 cells.CCK-8 assay was used to detect cell viability.Transwell was used to detect the ability of cell migration and invasion.Apoptosis was detected by FITC/PI assay.Western blot assay was used to analyze the changes of protein expression.Results:The results demonstrated the expression of KIFC1 was significantly higher in cholangiocarcinoma tissues than that in non-tumor tissues.The high expression of KIFC1 may be related to the poor prognosis of cholangiocarcinoma.The down-regulation of KIFC1 may inhibit the proliferation,migration and metastasis of cholangiocarcinoma cells and promote apoptosis by regulating PI3K/AKT pathway.Conclusion:KIFC1 promotes the malignant biological progress of cholangiocarcinoma,and its mechanism may be related to the regulation of PI3K/AKT pathway by KIFC1.KIFC1 may be a potential target to guide the diagnosis and treatment of cholangiocarcinoma.
作者
恽骁
王高超
周含煜
朱春富
秦锡虎
YUN Xiao;WANG Gaochao;ZHOU Hanyu;ZHOU Chunfu;QIN Xihu(Department of General Surgery, the Affiliated Changzhou NO.2 People's Hospital of Nanjing Medical University, Changzhou 213003,China;Department of Oncology Surgery,The Affiliated Changzhou NO.2 People's Hospital of Nanjing Medical University, Changzhou 213003,China)
出处
《东南大学学报(医学版)》
CAS
2021年第3期277-284,共8页
Journal of Southeast University(Medical Science Edition)
基金
国家自然科学基金资助项目(8167246)。