摘要
目的:采用生物信息学方法分析miR-130b-3p在肾透明细胞癌(KIRC)组织中的表达,预测其靶基因,并探究其预后价值,为进一步研究miR-130b-3p在KIRC中的调控机制和临床应用提供理论依据。方法:采用miRBase分析miR-130b-3p的保守性;dbDEMC2.0数据库评估miR-130b-3p在人肿瘤组织和正常组织中的表达,采用UCSC数据库的RNA-seq数据分析miR-130b-3p在KIRC组织中的表达;Stabase3.0和UALCAN数据库预测和确定miR-130b-3p在KIRC的靶基因,并采用Metascape对miR-130b-3p靶基因进行GO功能及KEGG通路富集分析;Kaplan-Meier Plotter分析miR-130b-3p表达与患者总生存期的关系,评估其在KIRC患者的预后意义。结果:miR-130b-3p序列在物种间高度保守,在肾癌(KDCA)等多种肿瘤中表达上调,且在KDCA高分级中较低分级明显上调;进一步分析其在KDCA最常见类型,即KIRC组织中的表达,结果显示,miR-130b-3p在KIRC组织中明显上调;预测获得miR-130b-3p在KIRC中的靶基因集包含87个靶基因;富集分析结果显示,靶基因主要富集于细胞自噬、顶树突发育、磷脂酰肌醇生物合成过程、肌动蛋白细胞骨架形成等生物过程和甲状腺激素合成、肌动蛋白细胞骨架调节及Ras等信号通路;生存分析显示,miR-130b-3p与KIRC患者不良预后显著相关。结论:miR-130b-3p在KIRC中表达异常,且涉及多个生物学过程和信号转导通路,可作为KIRC潜在的预后生物标志物。
Objective:To analyze expression of miR-130 b-3 p,to predict its target genes,as well as to explore its prognostic value in renal clear cell carcinoma(KIRC)by bioinformatics method,in order to provide theoretical basis for further studying regulatory mechanism and clinical application of miR-130 b-3 p in KIRC.Methods:miRBase to analyze conservativeness of miR-130 b-3 p.dbDEMC2.0 database to evaluate expression of miR-130 b-3 p in human cancer tissues and corresponding normal tissues,RNA-seq data from UCSC database to analyze expression of miR-130 b-3 p in KIRC.Stabase3.0 and UALCAN databases were performed to predict and determine target genes of miR-130 b-3 p in KIRC.GO function and KEGG pathway enrichment analysis of miR-130 b-3 p target genes were performed in Metascape.Kaplan-Meier Plotter to analyze relationship between miR-130 b-3 p expression and overall survival of patients and to evaluate its prognostic significance.Results:Sequence of miR-130 b-3 p was highly conserved among species.miR-130 b-3 p was up-regulated in various tumors including kidney cancer(KDCA),and was significantly up-regulated in high grade compared with low grade of KDCA.Further analyzed expression of miR-130 b-3 p in most common type(KIRC)of KDCA showed that miR-130 b-3 p was markedly up-regulated in KIRC.Target gene set containing 87 predicted target genes of miR-130 b-3 p in KIRC were obtained.Results of enrichment analysis suggested that target genes were mainly enriched in biological processes including cell autophagy,apical dendritic development,phosphatidyl inositol biosynthesis process,actin cytoskeleton formation and pathways including thyroid hormone synthesis,actin cytoskeleton regulation and Ras signaling pathway.Survival analysis indicated that miR-130 b-3 p was significantly associated with poor prognosis of patients with KIRC.Conclusion:miR-130 b-3 p was abnormally expressed in KIRC,and involved in multiple biological processes and signal transduction pathways,which might be used as a novel biomarker for prognosis predic
作者
邹元章
卢俅
陈兵海
ZOU Yuan-Zhang;LU Qiu;CHEN Bing-Hai(Department of Urology,Affiliated Hospital of Jiangsu University,Zhenjiang 212001,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2021年第13期1614-1618,共5页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81402100)资助。
