摘要
目的:基于网络药理学探讨通窍活血汤治疗颅脑损伤(TBI)的作用机制。方法:通过检索中药系统药理学数据库与分析平台(TCMSP)数据库筛选出通窍活血汤潜在活性成分及靶点,采用GeneCard与OMIM数据库获得颅脑损伤疾病靶点,得到通窍活血汤的中药与TBI的交集靶点,使用Cytoscape 3.7.2软件绘制活性成分靶点疾病交互网络图。采用String数据库构建靶点蛋白质-蛋白质相互作用(PPI)网络,并进行基因本体(GO)富集分析和京都基因和基因组百科全书(KEGG)信号通路富集分析。结果:筛选后收集得所有中药成分127个,收集疾病靶点1811个,疾病与药物交集靶点共62个。PPI网络提示核心靶点为ALB、IL6、CASP3、EGFR、VEGFA等;GO功能注释得到73条富集结果,包括DNA结合转录激活剂活性,RNA聚合酶Ⅱ特异性等,富集得到PI3K-AKT等75条核心的KEGG通路。结论:通窍活血汤治疗颅脑损伤具有多成分、多靶点、多通路的作用机制特点。
Objective:To explore the mechanism of Tongqiao Huoxue Decoction in the treatment of craniocerebral injury based on network pharmacology.Methods:The potential active ingredients and targets of Tongqiao Huoxue Decoction were screened by searching the TCMSP database,and the target diseases of brain injury were obtained using GeneCard and OMIM database,and the intersection target of the Chinese medicinal of Tongluo Huoxue Decoction and TBI intersection target were obtained.Cytoscape 3.7.2 software was used to draw the interactive network diagram of the active ingredient target disease.The String database was used to construct the target interaction PPI network,and the GO function enrichment analysis and KEGG signal pathway enrichment analysis were performed.Results:A total of 127 Chinese medicinal components were collected,1811 disease targets were collected,and a total of 62 disease and drug intersection targets were collected.The PPI network suggested that the core targets were ALB,IL6,CASP3,EGFR,VEGFA,etc.;GO function annotations yielded 73 enrichment results,including DNA binding transcription activator activity,RNA polymeraseⅡtransformation,etc.,enrichment yielded PI3K-AKT,etc.and 75 core KEGG pathways.Conclusion:Tongqiao Huoxue Decoction has the characteristics of multi-component,multi-target and multi-path mechanism in the treatment of craniocerebral injury.
作者
杜勇
张淑敏
王雷
王文鑫
东潇博
刘佳霖
蔡旭
陈嘉琪
王革生
DU YONG;ZHANG Shumin;WANG Lei;WANG Wenxin;DONG Xiaobo;LIU Jialin;CAI Xu;CHEN Jiaqi;WANG Gesheng(Dongfang Hospital Beijing University of Chine Medicine,Beijing 100078,China;Civil Aviation General Hospital,Beijing 100123,China;Beijing University of Chine Medicine,Beijing 100029,China)
出处
《世界中医药》
CAS
2021年第15期2282-2288,2292,共8页
World Chinese Medicine
基金
国家中医药管理局国家中医临床研究基地业务建设科研专项课题(JDZX2015043)——破血化瘀法治疗出血性中风急性期干预时点及风险的研究
北京中医药大学2018年度基本科研业务青年教师项目(2018-JYBZZ-JS145)——通窍活血汤对颅脑损伤后患者神经功能恢复及神经因子含量的实验研究。
关键词
通窍活血汤
颅脑损伤
网络药理学
靶点
蛋白互作网络
信号通路
富集分析
作用机制
Tongqiao Huoxue Decoction
Traumatic brain injury
Network pharmacology
Targets
Protein protein interaction network
Signal pathway
Enrichment analysis
Mechanism of action
