摘要
本研究基于荔枝转录组数据,对GA2ox(Gibberellin 2-oxidase)基因家族进行了鉴定和筛选,利用转录组数据及实时荧光定量PCR方法,分析不同品种和组织间的赤霉素合成途径关键酶LcGA2ox基因的表达情况。结果表明:转录组数据中共鉴定到9个荔枝LcGA2ox基因,均具有2OG-Fell_Oxy结构域;通过基因克隆,得到3条LcGA2ox(LcGA2ox1,LcGA2ox2和LcGA2ox3),开放阅读框全长分别为999、1023和1023 bp。氨基酸进化树比对分析结果显示,除LcGA2ox6与水稻OsGA2oxs聚在一起外,其余LcGA2oxs与拟南芥AtGA2oxs关系最近。通过基因表达分析发现,LcGA2oxs基因在矮化品种‘紫娘喜’顶芽和叶片中的表达量更高,有5个LcGA2ox基因(LcGA2ox1,LcGA2ox2,LcGA2ox5,LcGA2ox6,LcGA2ox9)在‘紫娘喜’顶芽样品中上调表达,LcGA2ox3和LcGA2ox4基因在‘紫娘喜’叶片中显著上调表达,LcGA2oxs基因表达具有一定的表达特异性,且在株型差异显著的两个荔枝品种中表达差异显著,推测LcGA2ox基因通过负调控赤霉素的合成从而影响荔枝株型的形成。本研究结果将为进一步分析LcGA2ox调控荔枝生长发育作用机制及生物学功能、荔枝矮化性状的分子鉴定以及矮化资源创新利用深入研究提供参考依据。
In this study,the GA2ox(Gibberellin 2-oxidase)gene family in litchi were analyzed and charactered,and the expression pattern of LcGA2oxs in the different varieties and tissues were analyzed using both the transcriptome data and real-time quantitative PCR(RT-qPCR)method.The results showed that 9 litchi LcGA2ox genes with 2OG-Fell_oxy domain were identified by transcriptome data.Three LcGA2ox genes(LcGA2ox1,LcGA2ox2 and LcGA2ox3)were cloned and verifie based on PCR technique,and the lengths of the responed open reading frame(ORF)were 999,1023 and 1023 bp,respectively.The results of amino acid phylogenetic tree analysis showed that,except LCGA2ox6 and OsGA2oxs clustered together,the other LCGA2oxs had the closest relationship with AtGA2ox of Arabidopsis thaliana.By the transcriptome data,most of the genes are significantly up-regulated in the'Zinianxi'samples.Five LcGA2ox genes(LcGA2ox1,LcGA2ox2,LcGA2ox5,LcGA2ox6,Lc-GA2ox9)are only up-regulate in ZNX apical-buds sample and two LcGA2ox genes(LcGA2ox3,LcGA2ox4)are only up-regulate in ZNX leave s sample.Real-time quantitative PCR analysis showed that LcGA2oxs had high expression in ZNX's samples,These results suggested tissue-specific expression patterns of GA2oxs family genes.In addition,there were significant differences in expression between the two litchi varieties,except that LcGA2ox1 has high expression in the leaves of FZX.LcGA2ox genes regulate the height of litchi plants negatively.The results of this study will provide reference for the mechanism and biological functions of LcGA2ox regulating the growth and development of litchi,and the molecular identification of litchi dwarfing traits and in-depth research on innovative utilization of dwarf resources.
作者
陈哲
王祥和
周文静
周瑞云
吴文碟
吴凤芝
范鸿雁
胡福初
Chen Zhe;Wang Xianghe;Zhou Wenjing;Zhou Ruiyun;Wu Wendie;Wu Fengzhi;Fan Hongyan;Hu Fuchu(Haikou Investigation Station of Tropical Fruit Trees,Ministry of Agriculture,Key Laboratory of Tropical Fruit Tree Biology of Hainan Province,Institute of Tropical Fruit Trees,Hainan Academy of Agricultural Science,Haikou,571100)
出处
《分子植物育种》
CAS
北大核心
2021年第14期4626-4636,共11页
Molecular Plant Breeding
基金
海南省重点研发计划项目(ZDYF2018236)
国家自然科学基金项目(31701885)
国家荔枝龙眼产业技术体系海口综合试验站(CARS-32-26)共同资助。
