摘要
超声作为一种常用的物理刺激手段,其机械作用在微观范围内对植物细胞的生长会产生一定程度的影响。为了研究超声对绿豆种子生长发育的影响,本研究通过理论分析及仿真设计得出植物矩形细胞的特征频率,并设计了一种超声波刺激装置对绿豆种子进行刺激。利用该装置分别研究了超声频率、刺激时间和正弦波信号激励电压对绿豆种子发芽指数的影响,并在此基础上研究了连续波和脉冲波超声以及超声协同不同浓度NaCl对绿豆发芽的影响。结果表明,脉冲波超声较优于连续波超声,当脉冲波占空比为20%时,在48 kHz、35 min、10 Vpp条件下绿豆发芽率最高,该频率与仿真计算的结果基本一致,而且体积摩尔浓度为17.09 mmol·L^(-1)的NaCl溶液协同超声处理可以极大地加速绿豆发芽。结果证明理论推导及仿真计算结果可信,也由此证明适当的超声和NaCl协同刺激可加快植物种子生长,提高发芽率。
As a common means of physical stimulation, the mechanical action of ultrasound can affect the growth of plant cells to a certain extent in the microscopic scope. In order to study the effect of ultrasonic on the growth and development of mung bean seeds, the characteristic frequency of rectangular cells was obtained by theoretical analysis and simulation design, and an ultrasonic stimulation device was designed to stimulate mung bean seeds. Meanwhile, the effects of ultrasonic frequency, stimulation time and sinusoidal signal excitation voltage on the germination index of mung bean seeds were studied, and the effects of continuous wave and pulse wave ultrasound and ultrasonic combined with different concentrations of sodium chloride on the germination index of mung bean seeds were also studied. The results show that pulse wave ultrasonic was better than continuous wave ultrasonic. The germination rate of mung bean was the highest under the conditions of 48 kHz, 35 min and 10 Vpp when the duty ratio of pulse wave was 20%. The frequency was basically consistent with the simulation results. Moreover, NaCl solution with a molar concentration of 17.09 mmol·L^(-1) combined with ultrasonic treatment could greatly accelerate the germination of mung beans. The results proved that the theoretical derivation and simulation results were reliable, and it was also proved that appropriate ultrasonic and NaCl co-stimulation could accelerate the growth of plant seeds and improve the germination rate.
作者
闫延鹏
郑杰
张雪梅
陈宇
李鸿淋
崔建国
YAN Yanpeng;ZHENG Jie;ZHANG Xuemei;CHEN Yu;LI Honglin;CUI Jianguo(School of Pharmacy&.Bioengineering,Chongqing University of Technology,Chongqing 400054,China;Chongqing Key Laboratory of Medicinal Chemistry&.Molecular Pharmacology,Chongqing University of Technology,Chongqing 400054,China)
出处
《种子》
北大核心
2021年第7期142-148,共7页
Seed
基金
国家科技支撑计划项目(2015 BAI 01 B 14)
重庆市高等教育教学改革研究一般项目(173114)
重庆理工大学研究生教育优质课程项目资助(yyk 2017106)
重庆市技术创新与应用示范(社会民生类)一般项目(cstc 2018 jscx-msyb 0290)。
关键词
超声
特征频率
绿豆种子
NACL
发芽指数
ultrasound
characteristic frequency
mung bean seed
NaCl
germination index
