摘要
目的建立测定复方氨酚美沙糖浆中对氨基酚含量的方法。方法采用高效液相色谱法,色谱柱为Agilent SB C18柱(250 mm×4.6 mm,5μm),流动相为0.05 mol/L醋酸铵溶液-甲醇,进行梯度洗脱,流速为1.0 ml/min,柱温为30℃,检测波长为231 nm。结果对氨基酚浓度在0.30~15.18μg/ml浓度范围内线性关系良好,r=1.000(n=6);平均回收率为100.30%(n=9),RSD为0.90%;检测限为0.1μg/ml,定量限为0.2μg/ml;耐用性良好。结论HPLC法测定复方氨酚美沙糖浆中对氨基酚含量简单、灵敏、结果准确,可用于复方氨酚美沙糖浆的质量控制。
Objective To establish a method to determine the content of para-aminophenol in compound paracetamol and dextromethorphan syrup.Methods Using high performance liquid chromatography,the chromatographic column was an Agilent SB C18 column(250 mm×4.6 mm,5μm),the mobile phase was 0.05 mol/L ammonium acetate solution-methanol,gradient elution was performed,and the flow rate was 1.0 ml/min.The column temperature was 30℃,and the detection wavelength was 231 nm.Results The concentration of p-aminophenol had a good linear relationship in the concentration range of 0.30~15.18μg/ml,r=1.000(n=6);The average recovery rate was 100.30%(n=9),the RSD was 0.90%;the detection limit was 0.1μg/ml,and the quantification limit was 0.2μg/ml;and the durability was good.Conclusion The determination of para-aminophenol in compound paracetamol and dextromethorphan syrup by HPLC is simple,sensitive and accurate,and it can be used for the quality control of compound paracetamol and dextromethorphan syrup.
作者
蔡锦雄
江坤
陈颜清
刘敏
李玉兰
CAI Jin-xiong;JIANG Kun;CHEN Yan-qing;LIU Min;LI Yu-lan(Shenzhen Institute for Drug Control/Shenzhen Key Laboratory of Pharmaceutical Quality Standards Research,Shenzhen 518057,Guangdong,China)
出处
《医学信息》
2021年第16期166-168,共3页
Journal of Medical Information
关键词
高效液相色谱法
复方氨酚美沙糖浆
对氨基酚
High performance liquid chromatography
Compound paracetamol and dextromethorphan syrup
Para-aminophenol
