摘要
为明确墨兰根腐病的病原,提出绿色生防措施,以发生根腐病的墨兰为材料,采用组织分离法分离病原菌,并对其进行形态学鉴定及分子生物学鉴定,通过柯赫氏法则验证致病性。同时,采集野外健康墨兰根部和土壤,采用稀释涂布法分离获得20株细菌,通过平板对峙培养,筛选出1株细菌MJ5-1能同时拮抗2株病原真菌。通过16S r RNA基因序列分析,该拮抗菌为短小芽孢杆菌(Bacillus pumilus)。结果表明,导致云南墨江栽培墨兰根腐病的病原是腐皮镰刀菌(Fusarium solani),短小芽孢杆菌(Bacillus pumilus)MJ5-1能抑制腐皮镰刀菌菌丝及孢子的生长。研究结果可为产业化栽培墨兰的根腐病诊断及无公害防治提供理论依据。
The paper aims to identify the pathogen of root rot and put forward green biological control measures. The pathogen of root rot of Cymbidium sinense was isolated by tissue isolation method, and identified by morphology and molecular biology. The pathogenicity was verified by Koch’s law. At the same time, 20 strains of bacteria were isolated from the roots and soil of wild healthy C. sinense by dilution coating method.Through plate confrontation culture, one strain of bacteria MJ5-1 was screened, which could antagonize two pathogens. The 16 S rRNA gene sequence analysis showed that the antagonistic strain was Bacillus pumilus.The results showed that Fusarium solani was the pathogen of root rot of cultivated C. sinense in Mojiang. B.pumilus MJ5-1 could inhibit the growth of mycelium and spore of F. solani. This study could provide a theoretical basis for the diagnosis and pollution-free control of root rot of cultivated C. sinense.
作者
唐婕
陈健鑫
尼玛此姆
魏玉倩
落追
韩雨庭
吕则佳
马焕成
伍建榕
Tang Jie;Chen Jianxin;Nima Cimu;Wei Yuqian;Luo Zhui;Han Yuting;Lv Zejia;Ma Huancheng;Wu Jianrong(Key Laboratory of Forest Disaster Warning and Control in Yunnan Higher Education Institutions/Southwest Forestry University,Kunming 650224;Key Laboratory of Biodiversity Conservation in Southwest China of State Forest Administration,Southwest Forestry University,Kunming 650224)
出处
《中国农学通报》
2021年第19期128-133,共6页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目“墨兰菌根真菌多样性与根腐病发生的抑病机理研究”(31860208)
国家自然科学基金项目“地生兰-菌根真菌-松三联共生关系研究”(31360198)
西南林业大学木棉纤维人工林产业化培育省创新团队培育项目(2018CH014)
云南省“云岭教学名师”培育项目。
关键词
墨兰根腐病
组织分离法
病原菌
鉴定
拮抗菌
筛选
root rot of Cymbidium sinense
tissue isolation method
pathogen
identification
antagonistic bacteria
screening
