摘要
目的:研究溪黄草药材的薄层鉴别及含量测定方法,提升溪黄草药材现有质量控制标准。方法:用薄层色谱法鉴别溪黄草药材中的迷迭香酸,利用高效液相色谱法测定齐墩果酸、熊果酸含量。结果:建立的薄层色谱方法能够准确定性溪黄草药材,HPLC方法能够准确测定溪黄草药材中齐墩果酸、熊果酸含量,加样回收率分别为99.78%(RSD=0.56%)、100.68%(RSD=0.67%)。结论:该方法操作简便、数据准确、重复性良好,可用于溪黄草药材的质量控制。
Objective:To improve the current standard of quality control for Xihuangcao by studying TLC identification and the method of determining of the contents of Xihuangcao.Methods:Rosmarinic acid in Xihuangcao was identified by TLC,and the contents of oleanolic acid and ursolic acid were determined by HPLC.Results:The established TLC method could accurately determine the chemical composition of Xihuangcao,HPLC method could accurately determine the contents of oleanolic acid and ursolic acid contained in Xihuangcao,average recovery rates were 99.78%(RSD=0.56%)and 100.68%(RSD=0.67%)respectively.Conclusion:The method,easy to operate,accurate and reproducible,could be used for quality control of Xihuangcao.
作者
马鹏文
师庆媛
朱昱澜
任一杰
MA Pengwen;SHI Qingyuan;ZHU Yulan;REN Yijie(Key Laboratory of Solid Dispersible Preparation of TCM in Gansu Province/Gansu Longshen Rongfa Pharmaceutical Company,Ltd.,Lanzhou 730102,China;Key Laboratory of Quality and Standard Research for Traditional Chinese Medicines of Gansu Province)
出处
《西部中医药》
2021年第7期51-54,共4页
Western Journal of Traditional Chinese Medicine
基金
甘肃省科技厅2017年科技重大专项计划(17ZD2FA009)。
