摘要
为了提高茶多酚中生物活性最强的多酚物质表没食子儿茶素没食子酸酯(EGCG)的稳定性,增强其抗龋齿作用,设计构建了一种含EGCG防龋齿磷脂纳米囊泡,利用纳米囊泡优良的载体性能和磷脂复合物解决EGCG稳定性问题,延长活性成分在牙齿表面吸附时间,增强EGCG的防龋齿疗效.采用紫外光谱扫描和差式扫描量热表征磷脂复合物中EGCG与磷脂的相互作用,测定了纳米囊泡的粒径、多分散系数(PDI)、包封率以及形貌评价了纳米囊泡的纳米特征,以变异链球菌评价了其抗致龋菌的效果.结果表明:EGCG在卵磷脂的脂肪酸链的包裹下,形成EGCG磷脂复合物,以此为基础构建的EGCG纳米囊泡粒径及PDI分别为119.36 nm和0.098,包封率达到(81.24±0.18)%,具有良好的稳定性,对变异链球菌具有较好的抑制作用,有望在口腔防龋领域中得到应用.
To increase the application in the control of oral anti-carie,the fabrication of phospholipid nanovesicles was designed to improve the stability of encapsulation Epigallocatechin gallate(EGCG).The phospholipid complex was characterized by ultraviolet spectral scanning and differential scanning calorimetry to explore the possibility of any interaction between EGCG and lecithin.EGCG-loaded nanovesicle was characterized for particle size,polydispersity coefficient(PDI),morphology and drug entrapment efficiency.Finally,the anti-caries activity of formulation was estimated to ensure its efficacy.The results showed that EGCG-loaded nanovesicles based on EGCG phospholipid complex had good stability and better inhibitory effect on streptococcus mutans.The particle size and PDI of EGCG-loaded nanovesicles were 119.36 nm and 0.098,respectively,and the encapsulation rate of EGCG-loaded nanovesiclea was(81.24±0.18)%.It was expected to be used in the field of human oral caries prevention.
作者
寇先勇
徐肖迪
张成
汪印元
张子林
祝红达
KOU Xianyong;XU Xiaodi;ZHANG Cheng;WANG Yinyuan;ZHANG Zilin;ZHU Hongda(College of Bioengineering and Food & Key Laboratory of Fermentation Engineering of Education Ministry & Hubei Key Laboratory of Industrial Microbiology,Hubei University of Technology, Wuhan 430068, China)
出处
《中南民族大学学报（自然科学版）》
CAS
北大核心
2021年第4期370-374,共5页
Journal of South-Central University for Nationalities：Natural Science Edition
基金
湖北省教育厅优秀中青年科技创新团队资助项目(T201705)
湖北工业大学大学生创新创业训练计划项目(201910500109)。
