摘要
番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)是烟草花叶病毒属新成员,番茄和辣椒是其主要寄主,被中国、欧盟、美国、澳大利亚、土耳其、新西兰、智利、阿根廷、俄罗斯、哈萨克斯坦、泰国等国家或地区纳入需检疫的有害生物。我们利用电镜观察、RT-PCR和SYBR Green实时荧光RT-PCR首次从进境的番茄种子检出ToBRFV。电镜观察显示该样品所携带的病毒粒子形态符合烟草花叶病毒属病毒的粒子特征,RT-PCR扩增出特异的条带,实时荧光RT-PCR有明显的扩增曲线,PCR产物测序结果与ToBRFV(GenBank登录号MK133093.1和KT383474.1)同源性高达99.65%。
Tomato brown rugose fruit virus(ToBRFV)is a recently described member of the Tobamovirus genus.Tomato and pepper are the main hosts.To prevent the introduction of ToBRFV,it has been required to quarantine by China,EPPO,the United States,Australia,Turkey,New Zealand,Chile,Argentina,Russia,Kazakhstan and Thailand,etc.We detected and identified ToBRFV from imported tomato seeds for the first time by electron transmission microscope,RT-PCR and SYBR Green real time RT-PCR.The result of observation showed that the virus particle morphology of the sample was consistent with the particle characteristics of Tobamovirus genus.RT-PCR amplied the specific band and SYBR Green real-time RT-PCR had obvious amplification curve and the sequencing results were 99.65%similar to ToBRFV sequence(GenBank login numbers MK133093.1 and KT383474.1).
作者
田沂民
俞禄珍
易建平
杨翠云
吴建祥
周雪平
于翠
Tian Yimin;Yu Luzhen;Yi Jianping;Yang Cuiyun;Wu Jianxiang;Zhou Xueping;Yu Cui(Technical Center for Animal,Plant and Food Inspection and Quarantine of Shanghai Customs District,Shanghai 200135,China;College of Agriculture and Biotechnology,Zhejiang University;Institute of Plant Protection,Chinese Academy of Agricultural Sciences)
出处
《植物检疫》
2021年第4期35-38,共4页
Plant Quarantine
基金
上海市科技兴农项目(2019-02-08-00-08-F01150)。
关键词
番茄褐色皱纹果病毒
番茄
种子
鉴定
tomato brown rugose fruit virus
tomato
seed
identification
