摘要
目的探讨β-连环蛋白(β-catenin)促进铜绿假单胞菌清除的机制。方法在过表达β-catenin的小鼠巨噬样RAW264.7细胞和骨髓来源巨噬细胞中,铜绿假单胞菌感染后,采用平板计数法检测细菌的清除情况;在过表达β-catenin的RAW264.7细胞和骨髓来源巨噬细胞中,铜绿假单胞菌感染前后,采用实时荧光定量聚合酶链反应(Real-time PCR)的方法检测抗菌肽LL-37基因的表达;在过表达β-catenin的RAW264.7细胞和骨髓来源巨噬细胞中,应用LL-37小干扰RNA(siRNA)沉默LL-37,铜绿假单胞菌感染后,采用平板计数法检测LL-37回复β-catenin介导的细菌清除作用。结果在RAW264.7细胞和骨髓来源巨噬细胞中,β-catenin促进铜绿假单胞菌的清除;在RAW264.7细胞和骨髓来源巨噬细胞中,铜绿假单胞菌感染后,β-catenin促进LL-37基因的表达;进一步实验发现,LL-37可回复β-catenin介导的细菌清除作用。结论β-catenin通过调控LL-37促进铜绿假单胞菌的清除。
Objective To investigate the mechanism ofβ-catenin in promoting to elimination Pseudomonas aeruginosa.Methods In over-expressedβ-catenin macrophage-like mouse RAW264.7 cells and bone marrow in over expressedβ-catenin RAW264.7 cells and bone marrow derived macrophages derived macrophages,the bacterial killing after Pseudomonas aeruginosa infection was counted by the plate count method;the antimicrobial peptide LL-37 gene expression before and after Pseudomonas aeruginosa infection was detected by adopting the Real-time PCR;in over-expressedβ-catenin RAW264.7 cells and bone marrow derived macrophages,after silencing LL-37 by LL-37 siRNA and Pseudomonas aeruginosa infection,the plate counting method was used to detect theβ-catenin mediated bacterial elimination effect reverted by LL-37.Resultsβ-catenin promoted the elimination of Pseudomonas aeruginosa in RAW264.7 cells and bone marrow derived macrophages;β-catenin promoted the expression of LL-37 gene in RAW264.7 cells and bone marrow derived macrophages after Pseudomonas aeruginosa infection;the further experiment found that LL-37 could revertβ-catenin-mediated bacterial elimination.Conclusionβ-catenin promotes the elimination of Pseudomonas aeruginosa via regulating LL-37.
作者
傅强
王伟佳
黄福达
缪丽韶
杨山虹
陈康
FU Qiang;WANG Weijia;HUANG Fuda;MIAO Lishao;YANG Shanhong;CHEN Kang(Laboratory Medicine Center,Zhongshan Municipal Peoples′Hospital,Zhongshan,Guangdong 528403,China)
出处
《检验医学与临床》
CAS
2021年第15期2173-2175,2179,共4页
Laboratory Medicine and Clinic
基金
广东省中山市社会公益科技研究专项资金立项项目(2018B1032)。
