摘要
目的:探讨川陈皮素(nobiletin,Nob)抑制高糖诱导心肌细胞肥大的作用及其机制。方法:利用高糖(high glucose,HG)刺激乳鼠心肌细胞(neonatal rat cardiomyocytes,NRCMs)建立心肌细胞肥大模型,并给予Nob、核因子E2相关因子2(Nrf2)抑制剂Brusatol干预,采用MTT法检测细胞存活率,qRT-PCR法检测C-myc、Nppa mRNA水平,免疫荧光检测心肌细胞表面积,Western blot法检测Nrf2和血红素加氧酶-1(HO-1)蛋白表达水平。结果:33.3 mmol/L HG刺激NRCMs 48 h,细胞存活率显著下降,C-myc、Nppa mRNA水平和细胞表面积显著升高,Nrf2和HO-1蛋白表达显著降低(P<0.05)。给予Nob干预后,与HG组比较,细胞存活率、Nrf2和HO-1蛋白表达显著上调,C-myc、Nppa mRNA水平和细胞表面积显著下降(P<0.05)。利用Brusatol抑制Nrf2活性,与HG+Nob组比较,上述指标被逆转。结论:Nob抑制高糖诱导的心肌细胞肥大,其机制可能与激活Nrf2/HO-1信号通路有关。
AIM:To investigate the effect of nobiletin(Nob)on cardiomyocyte hypertrophy induced by high glucose and its mechanism.METHODS:Neonatal rat cardiomyocytes(NRCMS)were stimulated with high glucose(HG)to establish cardiomyocyte hypertrophy and nobiletin was given.Cell viability was measured by MTT assay.C-myc and Nppa mRNA levels were detected by qRT-PCR.Cellular surface area was detected by immunofluorescence,and Nrf2 and HO-1 protein expressions were detected by Western blot.RESULTS:After stimulation with 33.3 mmol/L HG for 48 h,the survival rate of NRCMS was significantly decreased,C-myc,Nppa mRNA levels and cellular surface area were significantly increased,Nrf2 and HO-1 protein expression were significantly decreased(P<0.05).After Nob treatment,compared with HG group,cellular surface area,Nrf2 and HO-1 protein expression were significantly increased,C-myc and Nppa mRNA levels were significantly decreased.The above indexes were reversed by using Nrf2 inhibitor.CONCLUSION:Nob inhibits cardiomyocyte hypertrophy induced by high glucose,and its mechanism may be related to the activation of Nrf2/HO-1 signaling pathway.
作者
刘晓萍
赖香茂
欧阳资章
江晟
张莹
LIU Xiaoping;LAI Xiangmao;OUYANG Zizhang;JIANG Sheng;ZHANG Ying(Department of Pharmacy,the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital,Qingyuan 511518,Guangdong,China;Department of Urology,the Sixth Affiliated Hospital of Guangzhou Medical University,Qingyuan People's Hospital,Qingyuan 511518,Guangdong,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2021年第7期753-759,共7页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
广东省医学科研基金项目(A2021149)。
