摘要
目的探讨全身振动训练对股骨组织生物力学与Wnt3a蛋白表达的影响。方法取雌性SD大鼠48只,随机分为假手术组、骨质疏松组与全身振动组,每组16只。采用Micro-CT检测骨形态计量学参数,三点弯实验检测骨结构力学参数与材料力学参数,Western blotting检测Wnt3a、β-catenin蛋白表达量,qRT-PCR检测Wnt3a、β-catenin、cyclin D1、Tcf1基因表达量。结果与假手术组相比较,骨质疏松组骨密度、骨体积分数、骨小梁数量、骨小梁厚度及皮质骨厚度均降低,骨小梁间隙升高;与骨质疏松组比较,全身振动组骨密度、骨体积分数、骨小梁数量、骨小梁厚度及皮质骨厚度均升高,骨小梁间隙降低。与假手术组比较,骨质疏松组最大载荷、弹性载荷、最大挠度降低;与骨质疏松组比较,全身振动组最大载荷、弹性载荷、最大挠度升高。与假手术组比较,骨质疏松组最大应力、弹性应力、最大应变和弹性模量降低;与骨质疏松组比较,全身振动组弹性应力、最大应变和弹性模量升高。与假手术组比较,骨质疏松组Wnt3a、β-catenin蛋白及基因表达降低,cyclin D1、Tcf1基因表达量降低;与骨质疏松组比较,全身振动组Wnt3a、β-catenin蛋白及基因表达升高,cyclin D1、Tcf1基因表达量升高。结论全身振动训练可改善骨质疏松大鼠股骨生物力学性能,提高其Wnt3a蛋白表达。研究结果为全身振动训练防治骨质疏松提供了实验室参考数据。
Objective To investigate the effect of whole body vibration training on biomechanics and Wnt3 a protein expression of the femur. Methods Forty-eight female SD rats were randomly divided into sham operation group,osteoporosis group and whole body vibration group,16 in each group. The bone morphometric parameters were measured by Micro-CT,mechanical parameters of bone structure and materials were measured by three-point bending test,protein expression of Wnt3 a and β-catenin was measured by Western blotting,and gene expression of Wnt3 a,β-catenin,cyclin D1 and tcf1 was detected by qRT-PCR. Results Compared with sham operation group,bone mineral density( BMD),bone volume fraction( BVF),trabecular number,trabecular thickness and cortical bone thickness in osteoporosis group were decreased,and trabecular space was increased;compared with osteoporosis group,BMD,BVF,trabecular number,trabecular thickness and cortical bone thickness in whole body vibration group were increased,and trabecular space was decreased. Compared with sham operation group,the maximum load,elastic load and deflection of osteoporosis group were significantly reduced;compared with osteoporosis group,the maximum load,elastic load and deflection of whole body vibration group were significantly increased. Compared with sham operation group,the maximum stress,elastic stress,maximum strain and elastic modulus in osteoporosis group decreased significantly;compared with the osteoporosis group,the elastic stress,maximum strain and elastic modulus in whole body vibration group increased significantly.Compared with sham operation group,Wnt3 a,β-catenin protein and gene expression decreased,cyclin D1,tcf1 gene expression also decreased;compared with osteoporosis group, Wnt3 a, β-catenin protein and gene expression increased,cyclin D1,tcf1 gene expression increased as well. Conclusions Whole body vibration training can improve biomechanical properties of the femur and expression of Wnt3 a protein in osteoporotic rats.The research findings provide laboratory
作者
肖庆群
郑印渝
王亮
XIAO Qingqun;ZHENG Yinyu;WANG Liang(College of Physical Education,Guizhou Normal University,Guiyang 550018,China)
出处
《医用生物力学》
CAS
CSCD
北大核心
2021年第3期453-458,共6页
Journal of Medical Biomechanics
基金
贵州省体育局课题(GZTY2018202)
贵州省科技计划项目(黔科合基础[2017]1035)。
