摘要
Laser desorption ionization mass spectrometry(LDI-MS)is a primary tool for biological analysis.Its success relies on the use of chemical matrices that facilitate sof desorption and ionization of the biomolecules,which,however,also limits its application for metabolomics study due to the chemical interference by the matrix compounds.Te requirement for sample pretreatment is also undesirable for direct sampling analysis or tissue imaging.In this study,antirefection(AR)metal surfaces were investigated as sample substrates for matrix-free LDI-MS.Tey were prepared through ultrafast laser processing,with high light-to-heat energy conversion efciency.Te morphology and micro/nanostructures on the metal surfaces could be adjusted and optimized by tuning the laser fabrication process.Te super-high UV absorption at 97%enabled highly efcient thermal desorption and ionization of analytes.Te analytical performance for the matrix-free LDI was explored by analyzing a variety of biological compounds,including carbohydrates,drugs,metabolites,and amino acids.Its applicability for direct analysis of complex biological samples was also demonstrated by direct analysis of metabolites in yeast cells.
基金
Tis work was fnancially supported by the National Natural Science Foundation of China(Project No.21627807 and 51575309)
We also thank Bruker(Beijing)Scientifc Technology Co.,Ltd.for making their instruments available for experiments.
