摘要
To study whether the antibody against the testis form of the nuclear autoantigenic sperm protein(tNASP)could result in reproductive failure,we successfully cloned and expressed a 339-bp cDNA fragment of mouse tNASP(mtNASP).Using mouse as a model,recombinant mtNASP(rmtNASP)and a synthetic peptide,human tNASP393-408(htNASP393-408),were investigated for their antifertility effect.Active immunization with rmtNASP or the synthesized peptide raised high antibody titers in the immunized mice.Sperm-egg binding and fusion assay were carried out in 8-10-week-old BALB/c mice.Sperm-egg binding and in vitro fertilization of mouse oocytes were inhibited by co-incubation of zona-free mouse oocytes with capacitated mouse spermatozoa in the presence of varying concentrations of the antisera against rmtNASP.There was a significant antifertility effect in animals immunized with rmtNASP or the synthesized peptide.The effect on fertility in the mice immunized with the synthesized peptide was reversible.Our data indicate that active immunization with rmtNASP antigen may induce a strong antibody response that causes an inhibition of fertility.
基金
the National Natural Science Foundation of China(No.30470909 to Chen Xu,and No.30640031 to Chen Xu)
the Deng Shan Project of Shanghai Municipal Commission of Science and Technology(No.06JC14046 to Chen Xu)
the‘973’Basic Research Funding Scheme of China(No.G199905501 to Chen Xu)
the Science Research Project of Science&Technology Commission of Shanghai(No.06ZR14057 to Jian-Li Shi)
the Shanghai Leading Academic Discipline Project(No.S30201 to Chen Xu).
