摘要
目的探究血清基质金属蛋白酶9(MMP-9)和金属蛋白酶组织抑制剂2(TIMP-2)的表达水平对腹股沟疝行腹腔镜完全腹膜外修补术(TEP)后复发的预测价值。方法回顾性收集2016年1月至2020年6月亳州市人民医院外科确诊收治的80例腹股沟疝患者,行TEP术后进行为期6个月的随访,以是否复发将所有患者分为复发组11例和未复发组67例。采用采用酶联免疫吸附试验检测所有患者血清MMP-9和TIMP-2的表达水平,采用受试者工作特征(ROC)曲线判定各指标单独检测和联合检测的预测价值。结果手术前和术后1 d,2组患者血清MMP-9和TIMP-2表达水平差异无统计学意义(P<0.05);术后7 d和随访结束,复发组患者血清MMP-9[(139.23±18.21)、(192.17±23.17)μg/L]和TIMP-2[(133.85±13.25)、(153.14±19.47)μg/L]表达水平均显著高于未复发组[(103.65±11.96)、(90.19±12.82)μg/L;(106.24±11.46)、(71.39±14.32)μg/L],差异有统计学意义(P<0.05)。MMP-9单独检测对腹股沟疝术后复发预测的ROC曲线下面积(AUC)为0.723,敏感度为63.64%,特异度为71.79%;TIMP-2单独检测对腹股沟疝术后复发预测的AUC为0.715,敏感度为54.55%,特异度为66.67%;MMP-9和TIMP-2联合检测对腹股沟疝术后复发预测的AUC为0.878,敏感度为81.82%,特异度为83.33%,高于MMP-9或TIMP-2单独检测。结论血清MMP-9和TIMP-2的表达水平可作为早期预测腹股沟疝术后复发的重要指标,且2者联合检测具有更高的预测价值。
Objective To investigate the predictive value of combined detection of serum matrix metalloproteinase-9(MMP-9)and tissue inhibitors of metalloproteinases-2(TIMP-2)in predicting recurrence of inguinal hernia after totally extraperitoneal prosthesis(TEP)operation.Methods A total of 80 patients with inguinal hernia admitted to Bozhou People's Hospital from January 2016 to June 2020 were retrospectively collected.After 6 months'follow up,all patients were divided into recurrence group 11 cases and non-recurrence group 67 cases.The serum MMP-9 and TIMP-2 were detected by enzyme-linked immunosorbent assay,the predictive value were evaluated by receiver operating characteristic(ROC)curve.Results Before operation to 1 day after operation,there was no significant difference in serum MMP-9 and TIMP-2 expression levels between the two groups of patients(P<0.05);From 7 days after the operation to the end of the follow-up,the serum levels of MMP-9[(139.23±18.21),(192.17±23.17)μg/L]and TIMP-2[(133.85±13.25),(153.14±19.47)μg/L]in the relapsed group were significantly higher than those in the non-relapsed group[(103.65±11.96),(90.19±12.82)μg/L;(106.24±11.46),(71.39±14.32)μg/L],and the differences were statistically significant(P<0.05).ROC analysis showed that AUC,sensitivity and specificity of MMP-9 were 0.723,63.64%and 71.79%,the AUC,sensitivity and specificity of TIMP-2 were 0.715,54.55%and 66.67%,the AUC,sensitivity and specificity of MMP-9+TIMP-2 were 0.878,81.82%and 83.33%,which were higher than MMP-9 and TIMP-2.Conclusion The expression levels of serum MMP-9 and TIMP-2 were important factors in predicting recurrence of inguinal hernia postoperative,and combined detection of MMP-9 and TIMP-2 had higher predictive value.
作者
王雪雷
尹腾飞
谢磊
代景友
WANG Xue-lei;YIN Teng-fei;XIE Lei(Department of Pediatric Hernia Abdominal Wall,Bozhou People's Hospital,Bozhou Anhui 236800,China)
出处
《临床和实验医学杂志》
2021年第13期1406-1409,共4页
Journal of Clinical and Experimental Medicine
基金
国家卫生计生委医药卫生科技发展研究中心项目(编号:W2016EWAH07)。
关键词
腹股沟疝
基质金属蛋白酶9
金属蛋白酶组织抑制剂2
复发
预测价值
Inguinal hernia
Matrix metalloproteinase-9
Tissue inhibitors of metalloproteinases-2
Recurrence
Predictive value
