摘要
以有柄石韦(Pyrrosia petiolosa)为材料,制备有柄石韦叶柄、营养叶、孢子叶、孢子囊和去孢子叶5个部位的70%乙醇提取物和水提取物,测定其绿原酸(Chl)、芒果苷(Man)、咖啡酸(Caf)、芦丁(Rut)、总黄酮(TF)和总酚(TP)的含量以及抗氧化活性。结果表明:有柄石韦不同部位有效成分含量和抗氧化活性均有显著性差异(P<0.05);去孢子叶的总黄酮和总酚含量最高,分别为(129.46±4.03)mg/g和(47.85±2.98)mg/g,与营养叶的含量相似;有柄石韦各部位醇提物的抗氧化活性均高于水提物;各部位抗氧化活性强弱顺序为:营养叶>去孢子叶>孢子叶>叶柄>孢子囊;去孢子叶和营养叶的绿原酸和芦丁含量较高;营养叶的芒果苷和咖啡酸含量最高,显著高于其他4个部位(P<0.05);有柄石韦不同部位的ABTS+自由基清除能力、DPPH自由基清除能力及总抗氧化能力均与其有效成分含量呈正相关(r=0.69~0.98)。综合分析得知,有柄石韦营养叶的有效成分较多,具有较强的抗氧化活性,本研究结果可为石韦药材的开发利用提供科学依据。
The 70%ethanol extraction and water extraction solution were prepared from petioles,trophophylls,sporo-phylls,sporangia and sporophylls without sporangia of Pyrrosia petiolosa.The active ingredients and antioxidant activ-ity in the extraction,including chlorogenic acid(Chl),mangiferin(Man),caffeic acid(Caf),rutin(Rut),total flavonoids(TF),total phenols(TP)were assayed.There were significant differences among the five parts for active ingredients and antioxidant activity(P<0.05).TF and TP in sporophylls without sporangia were the highest,(129.46±4.03)mg/g and(47.85±2.98)mg/g respectively,which was similar to trophophylls.The antioxidant activity of ethanol extraction was higher than that of water extraction in each part.The antioxidant activity of each part was trophophylls>sporophylls without sporangia>sporophylls>petiole>sporangia.Chl and Rut in sporophylls without sporangia and trophophylls were higher.Man and Caf acid in trophophylls were the highest,which was significantly higher than that of the other four parts(P<0.05).Besides,the ABTS+free radical scavenging capacity,DPPH free radical scavenging abilities and total antioxidant capacity of different parts of P.petiolosa were positively correlated with active components(r=0.69~0.98).According to the comprehensive analysis,trophophylls had more effective components and stronger an-tioxidant activity.The study could provide scientific basis for the development and utilization of Pyrrosia.
作者
吴飘
宁珊
张凤媛
黄荣韶
李良波
姜学松
WU Piao;NING Shan;ZHANG Fengyuan;HUANG Rongshao;LI Liangbo;JIANG Xuesong(College of Agriculture,Guangxi University,Nanning,Guangxi 530004,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200,China;Jiangxi Hongxing Pharmaceutical Co.,Ltd,Fuzhou,Jiangxi 331800,China)
出处
《热带作物学报》
CSCD
北大核心
2021年第7期2059-2066,共8页
Chinese Journal of Tropical Crops
基金
广西创新驱动发展专项(桂科AA18118015)
江西红星药业有限公司委托项目。
关键词
有柄石韦
总黄酮
总酚
抗氧化活性
Pyrrosia petiolosa
total flavonoids
total phenols
antioxidant activity
