摘要
目的探讨四川地区自体精液保存患者精液细菌感染现状和细菌感染与精液质量、冷冻效果的关系。方法纳入2016年11月至2019年6月于四川大学华西第二医院精子库行自精保存的患者355例,分析其细菌感染情况。根据自精保存患者冷冻原因,将患者分为肿瘤患者及非肿瘤患者两类;再分别根据细菌感染情况,将患者分为细菌感染组及对照组,比较两类患者中细菌感染组与对照组的精液质量及冷冻效果。结果自精保存患者细菌感染率为12.68%,其中最常见细菌为阴道加德纳菌(46.67%),其次为B群链球菌(15.56%)、粪肠球菌(8.89%)及大肠埃希菌(4.44%)。肿瘤患者中,细菌感染组复苏后的平均浓度、前向运动精子比例(PR)及前向运动精子总数(TMC)均明显低于对照组(P<0.05);非肿瘤患者中,细菌感染组冷冻复苏后TMC明显低于对照组(P<0.05);在两类患者中,细菌感染组与对照组冷冻前精子质量差异无统计学意义(P>0.05)。结论自精保存患者细菌感染检出率较高为12.68%,细菌感染对精子冷冻有影响,可以降低复苏后TMC,尤其对肿瘤患者精液冷冻影响更大。
Objective To investigate the status quo of semen bacterial infection in patients with autologous semen cryopreservation in Sichuan Province and the relationship between bacterial infection,semen quality and cryopreservation effect.Methods A total of 355 patients who had their semen cryopreserved in the sperm bank of West China Second Hospital of Sichuan University from November 2016 to June 2019 were enrolled.Bacterial infection in semen was analyzed.Patients were divided into tumor patients and non-tumor patients according to the reasons for sperm cryopreservation.And patients were also divided into bacterial infection group and control group according to whether there was bacterial infection.Semen quality and cryopreservation effect were compared between bacterial infection group and control group in two types of patients.Results The bacterial infection rate of patients with autologous semen cryopreservation was 12.68%.The most common bacteria was Gardnerella vaginalis(46.67%),followed by group B streptococcus(15.56%),Enterococcus faecalis(8.89%)and Escherichia coli(4.44%).In tumor patients,the average sperm concentration,ratio of progressive motile sperm(PR),and total count of progressive motile sperm(TMC)after freeze-thaw in bacterial infection group were significantly lower than those in control group(P<0.05).In non-tumor patients,TMC after freeze-thaw in bacterial infection group was significantly lower than that in control group(P<0.05).There was no significant difference in sperm quality before cryopreservation between bacterial infection group and control group in two types of patients(P>0.05).Conclusion The detection rate of bacterial infection in patients with autologous semen cryopreservation is 12.68%.Bacterial infection has an effect on sperm cryopreservation,which reduces TMC after freeze-thaw,especially for tumor patients.
作者
鲜泱
蒋敏
刘博
赵文瑞
刘晓
柳莎莎
李福平
Xian Yang;Jiang Min;Liu Bo;Zhao Wenrui;Liu Xiao;Liu Shasha;Li Fuping(Human Sperm Bank, West China Second Hospital of Sichuan University, Chengdu 610041, China;Key Laboratory of Birth Defect and Related Diseases of Women and Children, Ministry of Education, Western Maternal and Child Reasearch Institute, Chengdu 610041, China)
出处
《成都医学院学报》
CAS
2021年第4期451-454,467,共5页
Journal of Chengdu Medical College
基金
四川省科技厅重点研发项目(No:2018FZ0043)。
关键词
自精保存
细菌感染
精液参数
肿瘤患者
冷冻效果
Autologous semen cryopreservation
Bacterial infection
Semen parameters
Cancer patients
Cryopreservation effect
