摘要
为探讨无机砷胁迫对铜藻(Sargassum horneri)的生理影响,对铜藻幼苗在不同浓度(0、50、100、150、200 mmol·L^(−1))的As^(3+)、As^(5+)短期胁迫条件下其氧化损伤、抗氧化酶、抗氧化物等8项生理指标进行了测定。结果显示,随着As^(3+)、As^(5+)浓度的增加,铜藻体内的氧自由基(Oxygen free radical,OFR)产生速率、丙二醛(Malondialdehyde,MDA)含量明显升高,铜藻细胞损伤率也同时上升。As^(3+)、As^(5+)对铜藻的半效应浓度(Median effect concentration,EC_(50))分别为50.1、116.3 mmol·L^(−1)。随As处理浓度升高,藻体超氧化物歧化酶(Superoxide dismutase,SOD)和过氧化物酶(Peroxidase,POD)活性呈上升趋势,而过氧化氢酶(Catalase,CAT)活性则无明显变化。谷胱甘肽(Glutathione,GSH)含量随As^(3+)浓度的增加呈先升后降,而随As^(5+)浓度的增加总体呈上升趋势。非蛋白巯基化合物(Non-protein thiol,NPT)含量随As^(3+)、As^(5+)浓度的升高而先升后降。对铜藻抗氧化系统主成分分析表明,As^(3+)50mmol·L^(−1)及As^(5+)100mmol·L^(−1)实验组的铜藻抗氧化综合得分较高;随着As^(3+)、As^(5+)浓度的升高,抗氧化物(GSH、NPT)对铜藻抗氧化系统的贡献减弱,而抗氧化酶(SOD、POD)对铜藻抗氧化系统的贡献相对增强。
To explore the impact of inorganic arsenic stress on physiology of Sargassum horneri,the 8 physiological indexes about oxidative damage,antioxidant enzymes and antioxidant were detected while the germlings of S.horneri were shortly treated with different concentrations(0,50,100,150,200 mmol·L^(−1))of As^(3+)and As^(5+),respectively.The results showed that rate of oxygen free radical(OFR)production and content of malondialdehyde(MDA)in S.horneri seedlings increased significantly with increasing concentration of As^(3+)or As^(5+),and the damage rate of cellular was also elevated simultaneously.The medium effect concentrations(EC_(50))of As^(3+)and As^(5+)to S.horneri were 50.1 and 116.3 mmol·L^(−1),respectively.Activities of superoxide dismutase(SOD)and peroxidase(POD)showed an upward trend in general,while catalase(CAT)varied insignificantly.The content of glutathione(GSH)increased first and then decreased with increase of As^(3+)concentration,whereas it showed an overall upward trend with increase of As^(5+)concentration.The content of non-protein thiol compound(NPT)tended to ascend first,then reduce with increasing As^(3+)or As^(5+)concentration.The principal component analysis of the S.horneri antioxidant system showed that the 50 mmol·L^(−1)(As^(3+))and 100 mmol·L^(−1)(As^(5+))treatments had higher comprehensive score of antioxidant system rather than others.As the concentration of As^(3+)and As^(5+)increased,the contribution of antioxidants(GSH,NPT)to the S.horneri antioxidant system weakened,while the contribution of antioxidase(SOD,POD)to the antioxidant system increased relatively.
作者
张鹏
刘玮
王铁杆
钟晨辉
陶月良
ZHANG Peng;LIU Wei;WANG Tiegan;ZHONG Chenhui;TAO Yueliang(Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource/Zhejiang Mariculture Research Institute,Wenzhou 325000,China;Algae Research Center,Marine Biology Institute of Shandong Province,Qingdao 266104,China;Key Laboratory of Cultivation and High-value Utilization of Marine Organisms in Fujian Province/Fisheries Research Institute of Fujian,Xiamen 361000,China;Wenzhou University,Wenzhou 325000,China)
出处
《生态环境学报》
CSCD
北大核心
2021年第5期1034-1041,共8页
Ecology and Environmental Sciences
基金
农业部藻类产业技术体系(CARS-50)
国家重点研发计划项目(2018YFC1406300)。
关键词
价态
抗氧化酶
抗氧化物
主成分分析
半效应浓度
valence state
antioxidase
antioxidant
principal component analysis
medium effect concentration
