摘要
目的观察针刺丰隆、足三里、内庭结合中药灌胃异功降脂散对高脂血症水平小鼠模型血脂的影响,探讨针药并举对高脂血症小鼠模型协同增效作用的机制。方法将SPF级KM雄性小鼠70只按照随机数字对照表随机分为空白组15只、模型组15只、辛伐他汀组10只、异功降脂散组10只、针刺组10只和针药组10只。空白组每日喂养普通饲料,其余5组通过每日喂养高脂饲料制备高脂血症小鼠模型。造模4周后,分组治疗并继续上述喂养。其中空白组、模型组灌胃10 mL/(kg·d)0.9%氯化钠;辛伐他汀组灌胃10 mL/(kg·d)辛伐他汀溶剂;异功降脂散组灌胃10 mL/(kg·d)异功降脂散;针刺组针刺双侧丰隆、足三里、内庭并灌胃10 mL/(kg·d)0.9%氯化钠;针药组针刺双侧丰隆、足三里、内庭并灌胃10 mL/(kg·d)异功降脂散。每日1次,治疗4周后摘取各组小鼠眼球采静脉血,分离出小鼠血清,测定各组小鼠的甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平。结果造模4周后,模型组与空白组相比TC、TG及LDL-C含量升高,HDL-C含量降低(P<0.05),提示高脂血症小鼠模型造模成功。治疗4周后,辛伐他汀组、异功降脂散组、针刺组、针药组与模型组相比TC、TG及LDL-C含量降低,HDL-C含量升高(P<0.05或P<0.01)。其中针药组TC、LDL-C含量明显低于异功降脂散组、针刺组,有统计学意义(P<0.05)。结论单纯针刺、单纯中药灌胃及针药结合都可减少高脂血症小鼠TC、TG,LDL-C,增加HDL-C,针药结合在减少TC、LDL-C方面效果更为明显,体现了针药并举较好的协同增效作用。
Objective To observe the effect of acupuncture at Zusanli(ST36),Fenglong(ST40) and Neiting(ST44) combined with intragastric administration of Yigong Jiangzhi Powder(异功降脂散) on blood lipids in hyperlipidemia mice and investigate the synergistic mechanism of acupuncture combined with Chinese herb on hyperlipidemia mice.Methods Seventy male KM mice of SPF grade were randomly divided into blank group(15 rats),model group(15 rats),western medicine group(15 rats),Chinese herb group(10 rats),acupuncture group(10 rats) and acupuncture combined with Chinese herb group(10 rats) according to random number comparative table.The blank group was fed with normal diet every day,the other five groups were made into hyperlipidemia model by feeding high-fat diet every day.After making model for 4 weeks,the mice were treated according to different groups and continued above feeding.The blank group and model group received gavage of 10 mL/(kg·d) physiological saline;the western medicine group received gavage of 10 mL/(kg·d) simvastatin solvent;the Chinese herb group received gavage of 10 mL/(kg·d) Yigong Jiangzhi Power;the acupuncture group received gavage of 10 mL/(kg·d) physiological saline,and then received acupuncture on both sides of Zusanli(ST36),Fenglong(ST40) and Neiting(ST44).Acupuncture combined with Chinese herb group received gavage of 10 mL/(kg·d) Yigong Jiangzhi Power,and then received acupuncture on both sides of Zusanli(ST36),Fenglong(ST40) and Neiting(ST44),1 time/d.After treatment 4 weeks,we enucleated the eyeballs of mice,collected venous blood,separated serum and measured the levels of triacylglycerol(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) of each group of mice.Results After making model for 4 weeks,compared with those of the blank group,the contents of TC,TG and LDL-C were increased and the contents of HDL-C were decreased in model group(P<0.05).It indicated that the model was successful.After treatment for 4 weeks,compare
作者
闫俐维
张志星
陈以国
YAN Liwei;ZHANG Zhixing;CHEN Yiguo(Liaoning University of Traditional Chinese Medicine,Liaoning,Shenyang 110847,Liaoning,China)
出处
《辽宁中医杂志》
CAS
2021年第6期225-228,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
辽宁省自然科学基金(20170540628)
国家自然科学基金(81072890)。
关键词
异功降脂散
高脂血症
针药并举
小鼠
丰隆
内庭
Yigong Jiangzhi Powder(异功降脂散)
hyperlipidaemia
acupuncture combined with Chinese herb
mice
Fen-glong(ST40)
Neiting(ST44)
