摘要
目的了解传染性单核细胞增多症(IM)患儿血清抗EB病毒(EBV)抗体谱类型,分析抗EBV衣壳抗原(VCA)免疫球蛋白(IgG)抗体亲合力检测在诊断IM中的价值。方法回顾性分析2016年5月至2019年5月在首都医科大学附属北京儿童医院诊断为IM的150例住院患儿的血清抗EBV抗体谱结果及血浆EBV核酸检测结果,抗EBV抗体谱包括抗VCA IgG和IgM,抗早期抗原(EA)IgA,抗EBV核抗原(EBNA)IgG和VCA IgG抗体亲合力。抗EBV抗体谱的检测方法为酶联免疫吸附法,血浆EBV核酸检测使用实时荧光定量PCR方法。结果150例IM患儿中抗EBV抗体谱大致可分为2种类型:1.抗VCA-IgM/IgG阳性、抗EBNA-IgG阴性和抗VCA-IgG低亲合力抗体阳性130例(86.7%),其中抗EA-IgA阳性50例;2.抗VCA-IgM阴性,抗VCA-IgG阳性、抗EBNA-IgG阴性和抗VCA-IgG低亲合力抗体阳性18例(12.0%),其中EA-IgA阳性5例。132例患儿进行了血浆EBV DNA检测,阳性率37.9%(50/132例)。结论儿童IM患者血清抗EBV抗体谱呈现多种类型,12.0%的IM患儿住院时抗VCA-IgM阴性,依靠抗VCA-IgG抗体亲合力检测可明确诊断。
Objective To understand the profile type of serum Epstein-Barr virus(EBV)antibodies in children with infectious mononucleosis(IM),and to analyze the significance of viral capsid antigen(VCA)IgG antibody affinity in the diagnosis of IM.Methods Retrospective analysis was performed on the results of the serum anti-EBV antibody profile and plasma EBV nucleic acid test of 150 hospitalized children with IM diagnosed in Beijing Children′s Hospital,Capital Medical University,from May 2016 to May 2019.Anti-EBV antibody profiles,including anti-VCA-IgG,anti-VCA-IgM,anti-early antigen(EA)IgA,anti-EBV nuclear antigen(EBNA)IgG,and anti-VCA-IgG affinity,were detected by enzyme-linked immunosorbent assay(ELISA).Plasma EBV nucleic acids were detected by real-time quantitative PCR.Results There were mainly two types of anti-EBV antibody profiles in 150 children with IM:(1)130 cases who were positive for anti-VCA-IgM/IgG,negative for anti-EBNA-IgG and positive for anti-VCA-IgG antibodies with low affinity,accounting for 86.7%(130/150 cases),of which 50 cases were positive for anti-early antigen IgA;(2)18 cases who were negative for anti-VCA-IgM,positive for anti-VCA-IgG,negative for anti-EBNA-IgG and positive for anti-VCA-IgG antibody with low affinity,accounting for 12.0%(18/150 cases),of which 5 cases were positive for anti-EA IgA.EBV DNA was measured in 132 children,with a posi-tive rate of 37.9%(50/132 cases).Conclusions There were several types of serum EBV antibody profiles in children with IM,12.0%of patients with IM in this study were negative for anti-VCA-IgM,and the diagnosis of IM was confirmed by the affinity of anti-VCA IgG.
作者
关晓蕾
闫静
赵秋红
李秋萍
姚佳宁
崔越
艾军红
王然
谢正德
Guan Xiaolei;Yan Jing;Zhao Qiuhong;Li Qiuping;Yao Jianing;Cui Yue;Ai Junhong;Wang Ran;Xie Zhengde(Beijing Key Laboratory of Pediatric Respiratory Infection Diseases,Key Laboratory of Major Diseases in Children,Ministry of Education,National Clinical Research Center for Respiratory Diseases,National Key Discipline of Pediatrics(Capital Medical University),Beijing Pediatric Research Institute,Beijing Children′s Hospital,Capital Medical University,National Center for Children′s Health,Beijing 100045,China)
出处
《中华实用儿科临床杂志》
CAS
CSCD
北大核心
2021年第13期1004-1006,共3页
Chinese Journal of Applied Clinical Pediatrics
