摘要
目的探究野黄芩素在结肠癌发生发展中的作用机制。方法分别应用MTT、流式细胞术、划痕、Transwell小室及小鼠荷瘤实验等方法,检测野黄芩素对结肠癌T84细胞增殖、凋亡、转移、侵袭及成瘤能力的影响;应用Western Blot技术检测野黄芩素对Bcl-2/Bax、caspase3/9、ERK1/2、p-ERK1/2、表皮生长因子受体(EGFR)、c-myc、c-fos蛋白表达的影响;应用免疫共聚焦技术检测野黄芩素处理后ERK1/2蛋白的亚细胞定位。结果野黄芩素处理可降低T84细胞的增殖活性、转移、侵袭及成瘤能力,促进细胞凋亡;降低EGFR、p-ERK1/2、c-myc及c-fos的表达,促进ERK1/2蛋白出核;过表达EGFR后,野黄芩素对T84细胞的增殖、成瘤的抑制作用及促进细胞凋亡作用明显降低(P<0.05)。结论野黄芩素可通过抑制EGFR/ERK1/2通路的激活抑制结肠癌的发生发展。
Objective To explore the effect mechanism of scutellarin in the occurrence and development of colon cancer.Methods MTT,flow cytometry,wound healing,Transwell chamber and mouse tumor-bearing test were carried out to determine the effect of scutellarin on the proliferation,apoptosis,migration,invasion and tumorigenesis of colon cancer T84 cells respectively.Western Blot was performed to assess the protein levels of Bcl-2/Bax,caspase3/9,ERK1/2,p-ERK1/2,epidermal growth factor receptor(EGFR),c-myc and c-fos after T84 cells were treated with scutellarin.Immunofluorescence technique was used to evaluate the subcellular location of ERK1/2 in T84 cells after scutellarin treatment.Results Scutellarin treatment inhibited the proliferation,migration,invasion and tumorigenesis of T84 cells,and induced cell apoptosis;scutellarin treatment decreased the expression of p-ERK1/2,EGFR,c-myc and c-fos,while translocated ERK1/2 from nuclear to cytoplasm.Inhibition of cell proliferation and tumorigenesis and enhancement of cell apoptosis caused by scutellarin were all weakened when EGFR was up-regulated in T84 cells.All differences were statistically significant(P<0.05).Conclusion Scutellarin represses the progression of colon cancer through inhibiting the activation of EGFR/ERK1/2 signaling pathway.
作者
李远志
王钧冬
岳朝驰
龙庆
李俊
LI Yuan-zhi;WANG Jun-dong;YUE Chao-chi;LONG Qing;LI Jun(Department of Traditional Chinese Medicine,the Clinical Academy of Southwest Medical University,the Affiliated Hospital of Southwest Medical University,Sichuan Province,Luzhou646000,China;Department of Gastroenterology,Hospital of Chengdu University of Traditional Chinese Medicine,Sichuan Province,Chengdu610000,China)
出处
《中国当代医药》
CAS
2021年第20期12-16,20,共6页
China Modern Medicine
基金
四川省科技厅科技计划项目(2018F20063)
四川省泸州市人民政府西南医科大学科技战略合作项目(2019LZXNYDJ26)。
