摘要
为分析大气细菌群落结构区域差异性,本研究采集了2014—2015年黄海、青岛和兰州大气生物气溶胶样品,对其进行了16S rRNA基因高通量测序分析,探讨环境因子(温度、风速和相对湿度)对大气细菌群落结构可能的影响。结果表明,3月黄海、青岛和兰州以及青岛不同采样时间的生物气溶胶中细菌群落结构在区域(p<0.01)和时间(p<0.05)分布上具有差异性,即黄海气溶胶中细菌群落多样性均高于陆地(p<0.01),3月青岛细菌群落的多样性高于兰州(p<0.01),且青岛春季大气生物气溶胶中细菌群落的丰富度和多样性高于冬季和夏季(p<0.05)。物种注释结果表明,变形菌(Proteobacteria)、厚壁菌(Firmicutes)、放线菌(Actinobacteria)和拟杆菌(Bacteroidetes)为这3个区域大气中的优势菌门。由于粗、细颗粒耐受环境变化的能力不同,不同区域粗粒径和细粒径样品的细菌群落的组成发生变化。例如,Bacteroides在黄海粗粒径样品中的相对丰度高于细粒径;Psychrobacter和Paenibacillus分别是3月青岛粗粒径和细粒径样品中的优势菌;沙尘发生时,兰州细粒径样品中的优势菌由Bifidobacterium转为Acinetobacter,粗粒径样品中的优势菌仍是Bifidobacterium但相对丰度值增大。典范对应分析表明,风速对细菌群落结构具有显著影响(p<0.01)。
In order to analyze the characteristics of atmospheric bacterial community structure in different regions,the 16S rRNA gene of bacterial in atmospheric bioaerosol samples collected from the Yellow Sea,Qingdao and Lanzhou from 2014 to 2015 is studied by high-throughput sequencing.The environmental factors(temperature,wind speed and relative humidity)influencing atmospheric bacterial community structure is also discussed in this paper.The results demonstrate that bacterial community diversity in aerosols of the Yellow Sea,Qingdao and Lanzhou in March and different sampling date in Qingdao have significant differences in regional(p<0.01)and temporal(p<0.05)distribution,respectively.In other words,bacterial community diversity in aerosols of the Yellow Sea are higher than those on land(p<0.01),and the diversity of bacterial communities in Qingdao in March are higher than that in Lanzhou(p<0.01),and the richness and diversity of bacterial communities in bioaerosols in Qingdao in spring are higher than in winter and summer(p<0.05).The species annotation results indicate that Proteobacteria,Firmicutes,actinobacteria and bacteroidetes are the dominant phly in the atmosphere of these three regions.Because the ability of coarse and fine particles to withstand changes of environment are different,bacterial community composition in the coarse and fine particle size samples have changed.For example,the relative abundance of Bacteroides in coarse particle size samples are higher than that of the fine particle size samples;Psychrobacter and Paenibacillus are the dominant bacteria in the coarse and fine particle size samples of Qingdao in March,respectively;When dust have occurred in Lanzhou,the dominant bacteria in the fine particle size samples change from Bifidobacterium to Acinetobacter,and the dominant bacteria in the coarse particle size samples are still Bifidobacterium but the value of relative abundance have increased.The canonical correspondence analysis(CCA)shows that wind speed has the significant effect on the bacte
作者
张波
马曼曼
孙宇航
冯文荣
米铁柱
甄毓
Zhang Bo;Ma Manman;Sun Yuhang;Feng Wenrong;Mi Tiezhu;Zhen Yu(Key Laboratory of Marine Environment and Ecology, Ministry of Education, Qingdao 266100, China;Laboratory for Marine Ecology and Environmental Science, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第9期80-92,共13页
Periodical of Ocean University of China
基金
国家自然科学基金项目(41775148)
中央高校基本科研业务费项目(201762006)
山东省自然科学基金项目(ZR2016CB47)资助。
关键词
生物气溶胶
细菌
群落结构
粒径
bioaerosol
bacteria
community structure
particle size
