摘要
目的了解江苏省无锡市2014―2016年随访的HIV感染者或AIDS患者(HIV/AIDS)HIV基因水平耐药变化特征。方法以2014年无锡市HIV/AIDS人群为研究对象,开展HIV分子流行病学调查及随访研究。收集感染者的基本信息,并从其血液样本中提取DNA,通过nest-PCR扩增pol基因片段并测序,将pol序列提交至HIV耐药数据库进行耐药突变位点分析。2016年随访相同人群,再次检测人类免疫缺陷病毒-1型(human immunodeficiency virus-1,HIV-1)耐药突变情况,分析两年间耐药变化情况。结果在2014―2016年共整理收集HIV/AIDS者612例,年龄主要集中在30岁及以上,占比85.46%(523/612),其中≥50岁人群比例较高,占比37.26%(228/612)。传播途径以同性性传播为主,占49.68%(304/612)。共检测1224例样本,成功获得811条pol序列,检出总耐药率为10.11%(82/811),对非核苷类反转录酶抑制剂(non-nucleoside reverse transcriptase inhibitors,NNRTIs)、核苷类反转录酶抑制剂(nucleoside reverse transcriptase inhibitors,NRTIs)和蛋白酶抑制剂(protease inhibitors,PIs)的耐药率分别为7.77%(63/811)、5.30%(43/811)和1.23%(10/811)。最常见的耐药突变位点是K103N/S和M184V/I。共有38例HIV/AIDS感染者随访前后耐药结果发生变化。结论无锡市HIV/AIDS感染者HIV-1基因水平耐药多样且复杂,耐药变化较大,应继续加强耐药突变监测及抗病毒治疗管理。
Objective To investigate the characteristics of HIV gene drug resistance changes among human immunodeficiency virus infected person/acquired immune deficiency syndrome patients(HIV/AIDS)in Wuxi City,Jiangsu Province from 2014 to 2016.Methods The HIV/AIDS population in Wuxi City in 2014 was used as the research object to carry out HIV molecular epidemiological investigation and follow-up research.Basic information of the infected persons were collected,and DNA was extracted from their blood samples.The pol gene fragment was amplified by nest-PCR and sequenced.The sequence was then submitted to the HIV drug resistance database for drug resistance mutation analysis.In 2016,the same population was followed up.The HIV-1 drug resistance mutation was tested again,and the change of drug resistance during two years was analyzed.Results A total of 612 HIV/AIDS cases from 2014 to 2016 were collected.Most of them were aged 30 or above,accounting for 85.46%(523/612),among which the proportion of people aged 50 or older was higher,accounting for 37.25%(228/612).The main route of transmission was homosexual transmission,accounting for 49.67%(304/612).A total of 1224 samples were tested and 811 pol sequences were successfully obtained.The total resistance rate was 10.11%(82/811).The drug resistance rates to non-nucleoside reverse transcriptase inhibitors,nucleoside reverse transcriptase inhibitors and protease inhibitors were 7.77%(63/811),5.30%(43/811)and 1.23%(10/811),respectively.The most common mutations were K103N/S and M184V/I.A total of 38 cases of HIV/AIDS had changes in drug resistance before and after follow-up.Conclusion The drug resistance of human immunodeficiency virus-1(HIV-1)gene level of HIV/AIDS patients in Wuxi was diverse and complex,and the drug resistance changed greatly.Therefore,the monitoring of drug-resistant mutations and the management of antiviral treatment should be strengthened.
作者
陈剑双
殷玥琪
成浩
张轩
袁德富
张晓轩
王晓敏
魏乾坤
王蓓
CHEN Jianshuang;YIN Yue-qi;CHENG Hao;ZHANG Xuan;YUAN De-fu;ZHANG Xiao-xuan;WANG Xiao-min;WEI Qian-kun;WANG Bei(Department of Epidemiology and Health Statistics,School of Public Health,Southeast University,Nanjing 210009,China;Department of Acute Infectious Diseases Prevention and Control,Xinwu District Center for Disease Prevention and Control,Wuxi 214028,China;Department of Disease Control,Wuxi Center for Disease Prevention and Control,Wuxi 214000,China)
出处
《中华疾病控制杂志》
CAS
CSCD
北大核心
2021年第6期709-715,共7页
Chinese Journal of Disease Control & Prevention
基金
江苏省研究生科研与实践创新计划(KYCX17_0184)
江苏省青年学生HIV-1感染传播的分子网络分析及社会网络探索(0701-184160070478)。
关键词
HIV感染者
艾滋病患者
耐药突变
HIV infected person
AIDS patients
Drug resistance variation
