摘要
目的探讨丙泊酚(PPF)通过线粒体凋亡通路对乳头状甲状腺癌TPC-1细胞凋亡和生长的影响。方法分别采用0、12.5、25、50μM丙泊酚处理TPC-1细胞,将细胞随机分为PPF 0μM组、PPF 12.5μM组、PPF 25μM组和PPF 50μM组进行后续实验。BrdU染色检测细胞增殖;克隆形成法检测细胞生长;流式细胞仪检测细胞凋亡;流式分选检测线粒体膜电位;蛋白免疫印迹检测Ki67、PCNA、Bcl-2、Bax、Caspase-3、Caspase-9、c-Myc蛋白表达水平;试剂盒检测SOD、MDA、GSH含量。结果与PPF 0μM组相比较,PPF 25μM组和PDF 50μM组BrdU阳性细胞数显著降低(P<0.05),克隆形成率显著降低(P<0.05),Ki67、PCNA蛋白水平显著降低(P<0.05),细胞凋亡率显著升高(P<0.05),细胞线粒体膜电位明显降低,Bax/Bcl-2、cleaved caspase-3/Caspase-3、cleaved caspase-9/Caspase-9比值显著升高(P<0.05),c-Myc蛋白水平显著降低(P<0.05),SOD含量显著降低(P<0.05),MDA、GSH含量显著升高(P<0.05)。结论丙泊酚激活线粒体凋亡通路诱导乳头状甲状腺癌TPC-1细胞凋亡和生长阻滞。
Objective To investigate the effect of propofol on the apoptosis and growth of tpc-1 cells in papillary thyroid cancer through mitochondrial apoptosis pathway.Methods TPC-1 cells were treated with 0,12.5,25,50μM doses of propofol,and randomly divided into PPF group 0μM,PPF group 12.5μM,PPF group 25μM and PPF group 50μM for subsequent experiments.BrdU staining was used to detect cell proliferation.Cell growth was detected by clone formation method.Cell apoptosis was detected by flow cytometry.Flow separation was used to detect mitochondrial membrane potential.Protein expression levels of Ki67,PCNA,bcl-2,Bax,caspase-3,caspase-9 and c-Myc were detected by western blot.The contents of SOD,MDA and GSH were detected by the kit.Results Compared with PPF group 0μM,the number of brdu-positive cells in PPF25μM and 50μM groups was significantly decreased(P<0.05),the clone formation rate was significantly decreased(P<0.05),the protein levels of Ki67 and PCNA were significantly decreased(P<0.05),the apoptosis rate was significantly increased(P<0.05),and the mitochondrial membrane potential was significantly decreased.The ratios of Bax/Bcl-2,cleaved caspase-3/caspase-3 and cleaved caspase-9/caspase-9 were significantly increased(P<0.05),the levels of c-myc protein were significantly decreased(P<0.05),the levels of SOD were significantly decreased(P<0.05),and the levels of MDA and GSH were significantly increased(P<0.05).Conclusion Propofol activates the mitochondrial apoptosis pathway and induces apoptosis and growth arrest of tpc-1 cells in papillary thyroid cancer.
作者
张子曦
王永顺
向洪聪
肖志波
夏学鑫
蔡志强
ZHANG Zixi;WANG Yongshun;XIANG Hongcong;XIAO Zhibo;XIA Xuexin;CAI Zhiqiang(Department of Anesthesiology, Jianghan Oilfield General Hospital, Qianjiang 433124, Hubei, China;Department of Anesthesiology, Qianjiang Central Hospital, Qianjiang 433100, Hubei, China;Department of Anesthesiology, Wuhan University of Science and Technology, Wuhan 430064, China)
出处
《西部医学》
2021年第7期976-981,共6页
Medical Journal of West China
基金
湖北省卫生计生委科研项目(WJ2017Q0022)。
关键词
乳头状甲状腺癌
丙泊酚
线粒体凋亡通路
氧化应激
Papillary thyroid carcinoma
Propofol
Mitochondrial apoptosis pathway
Oxidative stress
