摘要
目的建立一测多评法同时测定舒血宁注射液(银杏叶提取物)中4种银杏内酯的含量。方法该药物续滤液的分析采用TSK gel ODS⁃100V色谱柱(150 mm×4.6 mm,3μm);流动相水⁃甲醇,梯度洗脱;体积流量0.6 mL/min;柱温30℃。以银杏内酯A为内标,建立其他3种银杏内酯的相对校正因子,测定其含量。结果白果内酯、银杏内酯A、银杏内酯B、银杏内酯C在各自范围内线性关系良好(r>0.9900),平均加样回收率97.86%~100.79%,RSD 2.45%~3.07%。一测多评法所得结果与外标法接近。结论该方法简便灵敏,重复性好,可用于舒血宁注射液的质量控制。
AIM To establish a quantitative analysis of multi⁃components by single⁃marker(QAMS)method for the simultaneous content determination of four ginkgolides in Shuxuening Injection(Ginkgo Folium extract).METHODS The analysis of subsequent filtrate of this drug was performed on a 30℃thermostatic TSK gel ODS⁃100V column(150 mm×4.6 mm,3μm),with the mobile phase comprising of water⁃methanol flowing at 0.6 mL/min in a gradient elution manner.Ginkgolide A was used as an internal standard to calculate the relative correction factors of the other three ginkgolides,after which the content determination was made.RESULTS Bilobalide,ginkgolide A,ginkgolide B and ginkgolide C showed good linear relationships within their own ranges(r>0.9900),whose average recoveries were 97.86%-100.79%with the RSDs of 2.45%-3.07%.The results obtained by QAMS approximated those obtained by external standard method.CONCLUSION This simple,sensitive and reproducible method can be used for the quality control of Shuxuening Injection.
作者
游蓉丽
林珊
杨晓宁
高治华
姚静
YOU Rong-li;LIN Shan;YANG Xiao-ning;GAO Zhi-hua;YAO Jing(Institute of Basic Medicine of Traditional Chinese Medicine Clinic,China Academy of Chinese Medical Sciences,Beijing 100700,China;Shanghai State Institute of Pharmaceutical Industry,Shanghai 200433,China;Beijing Zhendong Guangming Pharmaceutical Research Institute Co.,Ltd.,Beijing 100085,China;Shanxi Zhendong Taisheng Pharmaceutical Co.,Ltd.,Datong 037010,China)
出处
《中成药》
CAS
CSCD
北大核心
2021年第7期1708-1712,共5页
Chinese Traditional Patent Medicine
基金
2019年山西省重点研发计划(社发领域)项目(201903D321208)。
关键词
舒血宁注射液
白果内酯
银杏内酯A
银杏内酯B
银杏内酯C
一测多评
Shuxuening Injection
bilobalide
ginkgolide A
ginkgolide B
ginkgolide C
quantitative analysis of multi⁃components by single⁃marker(QAMS)
