摘要
目的:检测子痫前期孕妇血清来源外泌体miR-30水平,分析其与促甲状腺素激素(TSH)和可溶性血管内皮生长因子受体-1(sFlt-1)水平关系以及在诊断子痫前期作用。方法:选择2018年1月-2020年6月本院诊治的子痫前期孕妇50例,其中25例轻度子痫前期、25例重度子痫前期;同期健康孕妇50例为对照组,检查血清来源外泌体miR-30、TSH和sFlt-1水平并分析3者相关性及对子痫前期的诊断价值。分析子痫前期危险因素。结果:重度子痫前期组miR-30(3.14±0.25)、TSH(5.32±0.51 uU/ml)和sFlt-1(5.47±0.48 ng/ml)表达水平高于轻子痫前期组(2.26±0.19、3.65±0.26 uU/ml、2.48±0.37ng/ml),且均高于对照组(1.02±0.15、2.13±0.18 uU/ml、1.11±0.22 ng/ml),子痫前期组miR-30与TSH和sFlt-1水平呈正性相关,子痫前期孕妇来源外泌体miR-30、TSH和sFlt-1水平与尿蛋白和血压呈正性相关(P<0.05)。logistic多因素回归分析血清来源外泌体miR-30、TSH和sFlt-1增加均是子痫早期独立危险因素(P<0.05)。血清来源外泌体miR-30联合TSH和sFlt-1对子痫早期诊断效能最佳,AUG值=0.81,敏感度70.1%、特异度64.0%。结论:血清来源外泌体miR-30、TSH和sFlt-1参与了子痫前期发病,具有作为子痫前期诊断标记物的可能。
Objective: To detect the level of serum exosomes miR-30 of pregnant women with preeclampsia, to study the relationship between the miR-30 level of the women and their levels of thyroid stimulating hormone(TSH) and soluble fms-like tyrosine kinase-1(sFlt-1), and to analyze the value of serum exosomes miR-30 level for diagnosing preeclampsia. Methods: 50 pregnant women with preeclampsia were selected in study group from January 2018 to June 2020, which included 25 women with mild preeclampsia in study group 1 and 25 women with severe preeclampsia in study group 2. 50 healthy pregnant women with singleton were randomly selected in control group during the same period. The levels of serum exosome miR-30, and TSH and sFlt-1 of the women in the three groups were detected, and the values of the levels of serum exosome miR-30, TSH, and sFlt-1 for diagnosing preeclampsia were analyzed. Results: The miR-30 expression level(3.14±0.25), the TSH level(5.32±0.51 uU/ml), and the sFLT-1 level(5.47±0.48 ng/ml) of the women in the study group 2 were significant higher than those(2.26±0.19, 3.65±0.26 uU/ml, and 2.48±0.37 ng/ml) of the women in the study group 1, and the miR-30 expression level, the TSH level, and the sFLT-1 level MiR-30 of the women in the study group were significant higher than those(1.02±0.15, 2.13±0.18 uU/ml, and 1.11±0.22 ng/ml) of the women in the control group. The miR-30 expression level of the women in the study group was positively correlated with their TSH level and sFLT-1 level. The serum exosomes miR-30, TSH, and sFLT-1 levels of the pregnant women with preeclampsia were positively correlated with their values of urine protein and blood pressure(P<0.05).Logistic multivariate regression analysis showed that the increase of serum exosome miR-30,TSH and sFLT-1 levels were the independent risk factors of preeclampsia early(P<0.05).The combination of serum exosome miR-30 level and the levels of serum TSH and sFLT-1 had the best diagnostic efficiency for preeclampsia,which’s AUG,sensitivity,and sp
作者
童婷婷
虞晴
尤子善
TONG Tingting;YU Qing;YOU Zishan(Suzhou Kowloon Hospital,Shanghai Jiaotong University School of Medcine,Jiangsu Province,215028)
出处
《中国计划生育学杂志》
2021年第6期1158-1162,共5页
Chinese Journal of Family Planning
基金
江苏省妇幼健康科研项目(F201847)
苏州市科学技术局(SYSD2019072)。
