摘要
Hepatocyte growth factor-regulated tyrosine kinase substrate(Hrs)is a key component of the endosomal sorting complexes required for transport and has been demonstrated to play a regulatory role in endocytosis/exocytosis and the accumulation of internal vesicles in multivesicular bodies.Citron kinase is a Ser/The kinase that we previously reported to enhance human immunodeficiency virus type 1(HIV-1)virion production.However,the relationship between Hrs and citron kinase in HIV-1 production remains elusive.Here,we report that Hrs interacts with citron kinase via its FYVE domain.Overexpression of Hrs or the FYVE domain resulted in a significant decrease in HIV-1 virion production.Depletion of Hrs by RNA interference in HEK293T cells increased HIV-1 virion production and enhanced the activity of citron kinase.These data suggest that Hrs inhibits HIV-1 production by inhibiting citron kinase-mediated exocytosis.
基金
in part supported by grants to Guangxia Gao from the National Natural Science Foundation of China(Grant No.81030030)
the Ministry of Science and Technology of China(No.2009zx09501-029).
