摘要
MicroProteins are small,5-15-kDa single-domain proteins that are evolutionarily related to multi-domain proteins with sequence homology(Eguen et al.,2015).The single domain of microProteins is often a protein-protein interaction(PPI)-domain,through which they can interact with their multi-domain protein targets(Figure 1).The first experimental insight that microProteins exist and how they act came from the identification of the regulatory feedback mechanism of class III homeodomain-leucine zipper(HD-ZIPIII)transcription factors by LITTLE ZIPPER(ZPR)microProteins(Wenkel et al.,2007;Kim et al.,2008).In Arabidopsis,the LITTLE ZIPPER microProtein family consists of four members(ZPR1-4)containing only a leucine zipper domain.The HD-ZIPIII transcription factor REVOLUTA directly transcriptionally upregu-lates multiple ZPR genes.ZPR proteins physically interact with their HD-ZIPIII targets and suppress their DNA binding ability.
基金
supported by a Charge de Researcher fellowship(no.19516174)
from the Fonds de la Recherche Scientifique,Belgium(to K.K.B.)
a Deutsche Forschungsgemeinschaft postdoc fellowship(to U.D.)
The laboratory of Stephan Wenkel is funded by grants from the independent Research Fund Denmark(0136-00015B and 0135-00014B)
the Novo Nordisk Foundation(NNF18OC0034226,NNF190C005658,and NNF200C0061440).
