摘要
目的统计分析来自中华骨髓库江苏分库8地市汉族造血干细胞志愿者HLA-B位点等位基因频率,比较各地市间的差异并分析常见HLA-B位点疾病易感基因在各地市的分布情况。方法采用聚合酶链反应-直接测序分型(PCR-SBT)技术,对中华骨髓库江苏分库共计27249名无关志愿者的HLA-B位点进行高分辨分型,按地市统计HLA-B位点等位基因频率并比较分析。结果江苏8地市汉族人群共检测出145种HLA-B位点等位基因,其中常州104种,南京113种,镇江83种,扬州88种,淮安88种,宿迁85种,徐州84种,盐城72种。总的来看,常州、扬州镇江非常接近,最常见的HLA-B位点等位基因依次均为B^(*)46∶01、13∶02、40∶01、58∶01;南京、淮安、宿迁比较接近,最常见的HLA-B位点等位基因依次均为B^(*)13∶02、46∶01、40∶01、58∶01、51∶01。盐城与南京、淮安、宿迁也比较接近,仅在B^(*)40∶01、58∶01的顺序上有所区别。徐州与南京、淮安、宿迁也比较接近,仅在B^(*)40∶01、46∶01的顺序上有所差异。结论本资料反应了江苏8地市汉族人群HLA-B位点等位基因分布状况,其分布规律与地域分布基本吻合。江苏8地市总体差异在于B^(*)13∶02与B^(*)46∶01频率的高低,苏北(淮安、宿迁、徐州、盐城)B^(*)13∶02的频率远高于B^(*)46∶01,南京B^(*)13∶02的频率略高于B^(*)46∶01,这点与我国北方一致;而苏南(常州)、扬州、镇江B^(*)13∶02的频率低于B^(*)46∶01,这点与我国南方一致。江苏8地市HLA-B位点等位基因多态性分析为研究HLA-B位点基因与疾病相关性以及江苏8地市人类基因遗传学提供了有意义的基础性资料。
Objective To investigate the frequencies of HLA-B alleles in Jiangsu Han population from Chinese Marrow Donor Program(CMDP) Jiangsu Branch in 8 prefecture-level cities, compare and analyze the distribution of common susceptible alleles at HLA-B locus in 8 prefecture-level cities. Methods The HLA-B locus of 27 249 unrelated volunteers from CMDP Jiangsu Branch were genotyped by polymerase chain reaction–sequence based typing(PCR-SBT) method, and frequencies of HLA-B alleles of 8 prefecture-level cities were calculated and compared. Results A total of 145 HLA-B alleles were detected in Jiangsu Han population, among them 104,113, 83, 88, 88,85,84,72 HLA-B alleles were detected in Changzhou, Nanjing, Zhenjiang, Yangzhou, Huai’an, Suqian, Xuzhou, and Yancheng Han population, respectively. In general, the common alleles in Changzhou, Yangzhou and Zhenjiang were very similar, and the most common alleles were B^(*)46∶01,13∶02,40∶01 and 58∶01. The common alleles in Nanjing, Huai’an and Suqian were rather similar, and the most common alleles were B^(*)13∶02,46∶01,40∶01, 58∶01 and 51∶01. The common alleles in Yancheng were rather similar to Nanjing, Huai’an and Suqian, with only difference in the order of B^(*)40∶01 and B^(*)58∶01. The common alleles in Xuzhou were close to Nanjing, Huai’an and Suqian too, with only difference in the order of B^(*)40∶01 and B^(*)46∶01. Conclusion The results reflected the distribution of HLA-B alleles of Jiangsu Han population from 8 prefecture-level cities and the gene distribution was basically consistent with the regional distribution. The major difference in HLA-B locus in Jiangsu 8 prefecture-level cities was B * 13∶02 and B * 46∶01. The frequency of B * 13∶02 in Northern Jiangsu(Huai’an, Suqian, Xuzhou, Yancheng) is much higher than that of B * 46∶01, and that in Nanjing is slightly higher than the frequency of B * 46∶01, which is consistent with that in North China. The frequency of B * 46∶01 in Southern Jiangsu(Changzhou), Yangzhou an
作者
潘芹芹
马骁
樊甦
王晓艳
尤雅洁
沈捷
PAN Qinqin;MA Xiao;FAN Su;WANG Xiaoyan;YOU Yajie;SHEN Jie(Department of HLA Lab,Jiangsu Province Hospital,the First Affiliated Hospital with Nanjing Medical University,Nanjing 210029 China)
出处
《中国输血杂志》
CAS
2021年第6期595-599,共5页
Chinese Journal of Blood Transfusion
关键词
HLA-B位点
等位基因频率
江苏8地市
HLA-B locus
alleles frequency
8 prefecture-level cities in Jiangsu
