摘要
背景腹部推拿作为传统的中医外治法之一,可以有效促进胃黏膜损伤修复,但其作用机制尚不明确.本研究假设腹部推拿作用于腹部后,可以通过脑-肠轴刺激下丘脑,并释放肠三叶因子(intestinal trefoil factor,ITF)作用于胃黏膜,进而发挥修复胃黏膜的作用.目的探讨腹部推拿促进胃黏膜损伤修复的作用机制,验证脑-肠轴在其过程中的桥梁作用.方法采用无水乙醇灌胃法复制胃黏膜损伤大鼠模型,将造模后大鼠随机分为腹部推拿组和模型组,另设空白对照组.腹部推拿组每天予推拿干预,模型组和空白对照组不进行任何干预.21 d后,采用ELISA法检测结肠组织和下丘脑中胃泌素(gastrin,GAS)、神经肽Y(neuropeptide Y,NPY)表达及血清中ITF含量,免疫组化法、Western Blot法检测下丘脑c-fos蛋白的表达,实时定量RT-PCR技术检测下丘脑和胃黏膜中ITF mRNA表达.结果模型组大鼠较空白对照组下丘脑组织中GAS和NPY含量均显著降低,结肠组织中GAS含量显著降低,NPY含量显著升高,下丘脑c-fos蛋白的表达显著增强,下丘脑及胃黏膜中ITF mRNA及血清中ITF水平降低;而经腹部推拿干预后结肠组织和下丘脑中GAS和NPY含量均有改善,下丘脑c-fos蛋白的表达显著降低,且下丘脑及胃黏膜中ITF mRNA和血清ITF水平显著增加.结论腹部推拿作用于胃黏膜损伤大鼠腹部可以改善结肠组织和下丘脑中GAS和NPY神经肽的含量,对中枢神经系统起到调控作用,验证了脑-肠轴的存在;且腹部推拿可以调节下丘脑c-fos蛋白表达,下调应激反应强度,同时刺激下丘脑释放ITF入血,并作用于胃黏膜,从而发挥修复胃黏膜的作用.
BACKGROUND As one of the external treatment methods of traditional Chinese medicine,abdominal massage can effectively promote the repair of gastric mucosal injury,but its mechanism is not clear.We hypothesized that abdominal massage can stimulate the hypothalamus through the brain-gut axis,result in the release of intestinal trefoil factor(ITF)to the gastric mucosa,and thus promote the repair of the gastric mucosa.AIM To explore the mechanism of abdominal massage to promote the repair of gastric mucosal injury,and to verify the role of the brain-gut axis in this process.METHODS Rats were randomly divided into an abdominal massage group,a model group,and a blank control groupp.A rat model of gastric mucosal injury was reproduced by absolute ethanol gavage.The abdominal massage group was given massage intervention every day,and the model group and the blank control group were not given any intervention.After 21 d,the expression of gastrin(GAS)and neuropeptide Y(NPY)in the colon and hypothalamus,and the content of ITF in serum were detected by ELISA.The expression of c-fos protein in the hypothalamus was detected by immunohistochemistry and Western blot.The expression of ITF mRNA in the hypothalamus and gastric mucosa was detected by real-time quantitative RT-PCR.RESULTS Compared with the blank control group,the contents of GAS and NPY in the hypothalamus were significantly decreased,the content of GAS in the colon was significantly decreased,the content of NPY in the colon was significantly increased,the expression of c-fos protein in the hypothalamus was significantly increased,and the content of ITF in the hypothalamus and gastric mucosa was significantly increased in the model group.After abdominal massage intervention,the contents of GAS and NPY in the colon and hypothalamus were improved,the expression of c-fos protein in the hypothalamus was significantly decreased,and the levels of ITF mRNA in the hypothalamus and serum ITF and gastric mucosa were significantly increased.CONCLUSION Abdominal massage can impr
作者
海兴华
刘芳
骆雄飞
李华南
张玮
王海腾
孙庆
Xing-Hua Hai;Fang Liu;Xiong-Fei Luo;Hua-Nan Li;Wei Zhang;Hai-Teng Wang;Qing Sun(First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300381,China)
出处
《世界华人消化杂志》
CAS
2021年第12期633-638,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金项目,No.81603711.
关键词
腹部推拿
胃黏膜损伤修复
脑-肠轴
肠三叶因子
Abdominal massage
Repair of gastric mucosal injury
Brain-gut axis
Intestinal trilobal factor
