摘要
目的:分析湿热瘀黄证慢加急性肝衰竭患者肠道菌群分布特征,探讨其在预测慢加急性肝衰竭预后中的价值。方法:纳入湿热瘀黄证慢加急性肝衰竭患者37例,提取粪便肠道菌群DNA,进行16S rDNA高通量测序,以入组24周时状况或死亡为终点判断,分为好转组和无好转组,应用生物信息分析软件和统计学软件对测序结果进行分析。结果:好转组患者厚壁菌门、芽孢杆菌纲、丹毒丝菌纲、乳杆菌目、巴斯德菌科、十八梭菌属的相对丰度高于无好转组(P<0.05),而变形菌门、肠杆菌目的相对丰度低于无好转组(P<0.05);两组间Alpha多样性分析没有明显差异;好转组患者肠道菌群物种数量大于无好转组;Lefse分析显示两组患者肠道菌群在多个分类学水平都有比较大的差异(P<0.05)。结论:湿热瘀黄证慢加急性肝衰竭患者病情进展与其肠道菌群有着密切联系,肠道菌群在预测病情转归及预后方面有着重要的价值。
Objective:Analysis the relationship between intestinal flora characteristics and the prognosis of damp-heat syndrome acute-on-chronic liver failure.To explore the predictive value of intestinal flora in patients of damp-heat syndrome acute-on-chronic liver failure.Methods:A total of 37 patients with damp-heat syndrome acute-on-chronic liver failure were recruited in this study.The excrements in the patients were collected to test the intestinal flora,and the fecal intestinal flora DNA was obtained by fecal bacterial genome DNA extraction kits.Judging from the condition at 24 weeks of enrollment,it was divided into an improved group and a non-improved group.The 16S rDNA of metagenomics sequencing technology was applied to analyze the intestinal flora.The biology and SPSS statistical software were applied to analyze the sequencing results.Results:The abundance of Firmicutes、Bacilli,Erysipelotrichia,Lactobacillales,Pasteurellaceae and Clostridium-XVIII in the improved group was much greater than not-improved group(P<0.05).Whereas the abundance of Proteobacteria and Lactobacillales decreased relative to not-improved group(P<0.05).There is no significant difference in alpha diversity analysis between the two groups.The number of intestinal flora in improved group was greater than that in not-improved group.Lefse analysis showed that the intestinal flora between the two groups was different at multiple taxonomic levels(P<0.05).Conclusion:Damp-heat syndrome acute-on-chronic liver failure are closely related to intestinal flora.Intestinal flora has important value in predicting prognosis of the liver failure.
作者
李春青
过建春
荀运浩
王薇薇
石伟珍
LI Chun-qing;GUO Jian-chun;XUN Yun-hao(Xixi Hospital of Hangzhou(Hangzhou,Zhejiang,310023),China)
出处
《中西医结合肝病杂志》
CAS
2021年第7期600-605,共6页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
浙江省中医药科技计划项目(No.2018 ZB109)
国家“十三五”科技重大专项(No.2018ZX10725506-002)
过建春名老中医专家传承工作室(No.GZS2020035)。
关键词
肝衰竭
湿热瘀黄证
肠道菌群
预后
chronic severe hepatitis B
syndrome of dampness heat and stasis yellow
intestinal flora
prognosis
