摘要
【背景】界面微生物群落组成研究日益成为微生态系统的研究热点之一。【目的】探究鄱阳湖河湖交错带枯水期水体细菌群落空间分布。【方法】选取鄱阳湖河湖交错带的典型采样点11个,现场采集水样,进行理化指标分析。运用扩增子测序获得了上述样品的16SrRNA基因序列数据。根据多样性指数和丰富度指数计算比较11个采样点样品的微生物群落多样性和丰富度。根据β多样性距离矩阵,利用相似度树状图对细菌群落进行聚类分析,研究鄱阳湖河湖交错带微生物分布差异。基于冗余分析(Redundancy Analysis,RDA)研究理化因子与微生物群落分布之间的关系。【结果】鄱阳湖河湖交错带水体为中性或微酸性水质、总氮含量在V类标准以上、总磷含量在Ⅱ、Ⅲ、Ⅳ类标准间波动。鄱阳湖河湖交错带水体细菌优势种组成大体相似,只是其相对丰度有所区别,其中门级微生物群落优势类群为:变形菌门(Proteobacteria,36.18%)、厚壁菌门(Firmicutes,22.18%)、拟杆菌门(Bacteroidetes,17.06%)、放线菌门(Actinobacteria,8.87%)和蓝细菌门(Cyanobacteria,4.43%)。水体中变形菌门以γ-变形菌为优势菌群,以β-变形菌为次优势菌群;属分类水平上,相对丰度最高的是放线菌门(Actinobacteria)的其他属类(11.4%)。乐安河(R9采样点)与鄱阳湖长江交汇处(H11采样点)的细菌α多样性指数较高,万家桥(N2采样点)、赣江鄱阳湖交汇处(W6采样点)和瑶湖(Y10采样点)的α多样性指数最低。从细菌群落组成看:鄱阳湖大湖池保护区(W7采样点)、乐安河(R9采样点)和鄱阳湖长江交汇处(H11采样点)群落组成相似,其余8个采样点群落组成相似。pH值(P=0.7352)、总氮(P=0.7614)和总磷(P=0.6128)是影响微生物群落分布的主要环境因子。【结论】鄱阳湖河湖交错带枯水期水体细菌群落空间分布差异较为明显,其空间地理差异叠加人类活动的扰动,影响了植�
[Background] The microbial community composition at the interface has become one of the research hotspots in the field of microecosystem. [Objective] The objective of the study is to explore the spatial distribution of bacterial community river-lake ecotone of Poyang lake during dry season. [Methods] Eleven typical sampling points in the Poyang lake river-lake ecotone were selected to collect water samples for analyzing physical and chemical indicators. The 16 S rRNA gene sequence data of the above samples were obtained by PCR. The diversity and richness of microbial communities were calculated and compared of 11 sampling sites. According to the Beta diversity distance matrix, the similarity tree diagram was used to cluster the samples to explore the microbial distribution in the Poyang lake river-lake ecotone. The relationship between physical and chemical factors and microbial community distribution was studied based on redundancy analysis(RDA). [Results] The Poyang lake river-lake ecotone water was slightly acidic, and TN content exceeded standard V, and the content of TP fluctuated between the standards of II, III and IV. The composition of dominant species of bacteria in the interlaced zone of Poyang lake is roughly similar except the difference of relative abundance. Microbial community structure at phylum level was Proteobacteria(36.18%), Firmicutes(22.18%), Bacteroidetes(17.06%) and Actinobacteria(8.87%). The Proteobacteria in the water was dominated by γ-Proteobacteria, and the β-Proteobacteria was the sub-dominant flora. At the genus level, other genera of Actinobacteria had the highest relative abundance(11.4%). The microbial diversity index(Ace, Chao1, Shannon and Simpson) of Le?an River(R9) and confluence zone of Poyang lake and Yangtze river(H11) was higher than others, Wanjia bridge(N2), confluence zone of Poyang lake and Ganjiang river(W6) and Yaohu lake(Y10) microorganisms has the lowest diversity index. The composition of microbial community was divided into two categories. The composition of
作者
黄小兰
简正军
叶长盛
贾伟
HUANG Xiaolan;JIAN Zhengjun;YE Changsheng;JIA Wei(School of Geosciences,East China University of Technology,Nanchang,Jiangxi 330013,China;School of Geography and Environment,Jiangxi Normal University,Nanchang,Jiangxi 330022,China;Shandong Bio-Form Ecological Environment Co.Ltd.,Tai’an,Shandong 271000,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2021年第6期1862-1873,共12页
Microbiology China
基金
国家自然科学基金(42061041,41561002)
东华理工大学博士科研启动基金(DHBK2019404)。
关键词
鄱阳湖
河湖交错带
枯水期
细菌群落
空间分布
Poyang lake
river-lake ecotone
dry season
microbial community
spatial distribution
