摘要
该研究以6个生长期的五味子为研究对象,采用UPLC,GC及MS色谱技术检测其所含物质基础的种类和含量变化,包括木脂素、核苷、香气成分和脂肪酸类成分。从性状上来看,不同生长期五味子的质地、颜色、味等均不相同。从物质基础上来看,UPLC-MS同时检测12种木脂素类成分,8月末9月初样品中总木脂素的质量分数较高,其中,五味子醇甲最高达0.67%±0.01%,五味子醇乙、当归酰戈米辛H和五味子乙素次之,总趋势随五味子成熟则含量上升。UPLC检测13种核苷类成分,7月末样品总核苷含量最高,其中尿苷、鸟苷含量较高,成熟后降低。香气成分和脂肪酸采用GC-MS检测,共鉴定出53种香气成分,在8月末样品中香气成分总含量最高,其中,衣兰烯含量较高,香柑油烯次之;脂肪酸共检出24种,8月份果实基本成熟,样品中脂肪酸含量最高,其中,亚油酸含量居首位,油酸次之。综上,五味子果实的成熟程度与各类物质基础的含量和种类相关,生长期对五味子的品质有不同程度的影响,应根据目标成分含量变化规律,确定适宜的采收时间。同时,该研究结果可为五味子物质基础的质量评价提供参考。
Schisandrae Chinensis Fructus in six growth stages was taken as materials to study the species and content changes of material basis, which were detected by UPLC, GC and MS chromatography, including lignans, nucleosides, aroma components and fatty acids. The results showed that the texture, color and taste of Schisandrae Chinensis Fructus in six growth stages were different. On the material basis, 12 lignans were detected by UPLC-MS, and the content of total lignans was higher in the samples from late August to early September, among which the highest content of schisandrin was 0.67%±0.01%, followed by schizandrol B, angeloylgomisin H and schisandrin B, and the total content increased with the maturity of Schisandrae Chinensis Fructus. Thirteen kinds of nucleosides were detected by UPLC. The total nucleoside content was the highest in late July samples, in which the contents of uridine and guanosine were higher and decreased after maturity. Aroma components and fatty acids were identified by GC-MS. A total of 53 aroma components were detected and the highest total content was appeared in late August samples, of which ylangene was higher and bergamotene was followed. A total of 24 kinds of fatty acids were detected. The fruits matured basically in August, and the content of fatty acids in the samples was the highest, among which linoleic acid content was top the list and oleic acid was the second. To sum up, the maturity of Schisandra chinensis fruit is related to the content and variety of various material bases, and the growth period has different influences on the quality of Schisandrae Chinensis Fructus. Therefore, the appropriate harvesting time should be determined according to the change law of target components. The results of this study can provide reference for the quality evaluation of Schisandrae Chinensis Fructus material basis.
作者
宋明杰
滕璇
刘畅
李志满
孙印石
SONG Ming-jie;TENG Xuan;LIU Chang;LI Zhi-man;SUN Yin-shi(State Local Joint Engineering Research Center of Ginseng Breeding and Application,College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China;Institute of Special Animal and Plant Sciences,China Academy of Agricultural Sciences,Changchun 130112,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第9期2245-2253,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年基金项目(81803733)
吉林省教育厅“十三五”科学技术研究规划项目(JJKH20190948KJ)。
