摘要
为了解辣椒中NHX基因家族生物信息学功能,探究其在非生物胁迫下基因表达特性,进而为辣椒CaNHX基因的功能开发以及辣椒耐盐抗旱育种基因提供基础。通过生物信息学方法对辣椒NHX基因家族进行鉴定,并对基因结构、结构域、系统进化关系、基因表达模式和非生物胁迫表达情况进行分析。结果表明,在辣椒中有7个GME家族成员,可分为3个亚组,同时均包含Na^(+)/H^(+)exchange保守结构域;主要分布在5条染色体上,其基因大小之间差异明显,氨基酸数目维持在198~952个,外显子含量在7~21个。除CaNHX5外其余蛋白均不具有信号肽,为疏水性蛋白,定位在液泡的可能性最高,都属于跨膜蛋白;Motif分析发现,N端含有CXC24XC的锌指结构,C端含有Trp(W)-24和TrKA-N,在序列中高度保守,二级结构主要以不规则卷曲和α-螺旋为主。进化树分析表明,该基因编码的氨基酸序列与番茄亲缘关系最近。qRT-PCR分析结果表明,在渗透和盐胁迫下,CaNHX6的基因表达量显著增加,分别是0 h时的13.5,13.6倍;在茉莉酸甲酯和赤霉素处理后,CaNHX5和CaNHX6在辣椒叶片中表达量均呈显著上调趋势,其余则相反。不同的表达情况表明其在非生物胁迫中发挥作用。
To understand the bioinformatics function of the NHX gene family in pepper,explore its gene expression characteristics under abiotic stress,and provide a basis for the function development of pepper CaNHX gene and the pepper salt and drought resistance breeding genes.The pepper NHX gene family was identified by bioinformatics methods,and the gene structure,domains,phylogenetic relationships,gene expression patterns and abiotic stress expression were analyzed.The results showed that there were 7 GME family members in pepper,which could be divided into 3 subgroups,and all contained Na+/H+exchange conserved domains;they were mainly distributed on 6 chromosomes,and their gene sizes had obvious differences.The number was mainly maintained between 198-952,and the exon content was between 7-21.All proteins except CaNHX5 had no signal peptides and were hydrophobic proteins,which were most likely to be located in the vacuole.They were all transmembrane proteins;Motif analysis found that the N-terminal contained the zinc finger structure of CXC24XC,and the C-terminal contained Trp(W)-24 and TrKA-N,the highly conserved secondary structure in the sequence was mainly irregular coils andα-helices.Multi-sequence evolutionary tree construction analyses indicated that aminoacid sequence encoded by this gene was closely related to tomato.The results of qRT-PCR analysis showed that under osmotic and salt stress,the gene expression of CaNHX6 increased significantly,which was 13.5 and 13.6 times that at 0 h,respectively;after treatment with methyl jasmonate and gibberellin.The expression of CaNHX5 and CaNHX6 in pepper leaves showed a significant up-regulation trend,while the others were on the contrary.The different expressions indicated that they played a role in abiotic stress.
作者
罗建
许春苗
张国斌
郁继华
LUO Jian;XU Chunmiao;ZHANG Guobin;YU Jihua(College of Horticulture,Gansu Agriculture University,Lanzhou 730070,China;Gardening Workstation in Anding District,Dingxi City,Dingxi 743000,China)
出处
《华北农学报》
CSCD
北大核心
2021年第3期15-24,共10页
Acta Agriculturae Boreali-Sinica
基金
甘肃省干旱生境作物学重点实验室主任基金项目(GHSJ-2020-Z3)
甘肃省自然科学基金项目(17JR5RA156)。
