摘要
为明确过氧化氢浸种对低温条件下花生发芽及种子生理代谢的影响,以花生品种开农176为材料,测定了过氧化氢浸种后的种子发芽及相关生理指标。结果表明,过氧化氢浸种显著提高了花生的发芽能力,发芽势从0提高到24.45%,发芽率从39.33%提高到90.99%。过氧化氢提高了花生种子赤霉素含量,同时降低了脱落酸含量,发芽0、24、48 h的赤霉素含量分别比对照提高10.82%、5.73%、18.64%,脱落酸含量分别比对照降低44.98%、36.45%、39.70%。同时,提高了抗氧化酶SOD、CAT的活性以及可溶性糖、可溶性蛋白的含量,降低了丙二醛的含量;促进了大分子贮藏物质脂肪、蛋白质、淀粉的分解代谢,为种子发芽提供更多的ATP和蛋白质、核酸的合成底物。研究表明,H2O2通过介导抗氧化酶、ABA和GA以及贮藏物质的分解来促进低温胁迫下花生种子的萌发。
To investigate the effects of hydrogen peroxide soaking on peanut germination and seed physiological metabolism,the seed germination and related physiological indexes after seed soaking were determined using peanut variety Kainong 176 as experimental material.The results showed that the germination vigor of peanut was increased from 0 to 24.45%,and the germination percentage was increased from 39.33%to 90.99%by hydrogen peroxide soaking.Hydrogen peroxide treatment increased the content of gibberellin in peanut seeds and decreased the content of abscisic acid at germination stage.The contents of gibberellin in germinating seeds at 0,24,and 48 hour(s)were 10.82%,5.73%,and 18.64%higher than those of control,and the contents of abscisic acid were 44.98%,36.45%,and 39.70%lower than that of control,respectively.At the same time,hydrogen peroxide treatment enhanced the activities of antioxidant enzyme SOD and CAT,increased the contents of soluble sugar and soluble protein,decreased the content of MDA,promoted the catabolism of macromolecular storage substances such as fat,protein,and starch,and provided more ATP and substrates for protein and nucleic acid synthesis.Studies revealed that hydrogen peroxide could promote peanut seed germination under low-temperature stress by mediating antioxidant enzymes,ABA and GA,and storage matter decomposition.
作者
郝西
崔亚男
张俊
刘娟
臧秀旺
高伟
刘兵
董文召
汤丰收
HAO Xi;CUI Ya-Nan;ZHANG Jun;LIU Juan;ZANG Xiu-Wang;GAO Wei;LIU Bing;DONG Wen-Zhao;TANG Feng-Shou(Industrial Crops Research Institute,Henan Academy of Agricultural Sciences/Key Laboratory of Oil Crops in Huanghuaihai Plains,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crops Improvement,Zhengzhou 450002,Henan,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第9期1834-1840,共7页
Acta Agronomica Sinica
基金
国家重点研发计划项目“大田经济作物优质丰产的生理基础与调控”(2018YFD1000900)
河南省现代农业产业技术体系项目(S2012-5)资助。
关键词
花生
浸种
过氧化氢
发芽
生理代谢
peanut
soaking
hydrogen peroxide
germination
physiological metabolism
