摘要
Controlling inflammation meanwhile facilitating tissue regeneration has been considered as a promising strategy to treat inflammatory bone defect. Herein, we describe the synthesis of a bio-sensitive poly(lactic-co-glycolic acid)/mesoporous silica nanocarriers core-shell porous microsphere(PLGA/MSNsPMS) encapsulated poly(L-lactic acid)(PLLA) spongy nanofibrous micro-scaffold as a new generation of therapeutic platform for effective reconstruction of bone defects caused by periodontal diseases.The PLGA/MSNs-PMS were designed as stimuli-responsive carriers for on-demand co-delivery of multiple biomolecules to provide proper physiological environment, while the multi-level(from macro-,micro-to nanometers) nanofibrous and porous structures in PLLA micro-scaffold were in charge of the reconstruction of ECM, which synergistically contribute to the enhancement of new tissue formation under inflammatory condition. After local injection into periodontal tissue, this construct could sequentially release bone growth factor(BMP-2) as well as anti-inflammatory drug(celecoxib) loaded MSNs in response to the over-expressed matrix metalloproteinases(MMP) in periodontal region. During alveolar bone regeneration induced by BMP-2 and ECM like structure, the MSNs would further deliver celecoxib in target cells to achieve inflammation inhibition, resulting in effective treatment of periodontal disease.
基金
supported by the National Natural Science Foundation of China (81601606 to X. C.)
the Knowledge Innovation Program of Shenzhen (JCYJ20170816100941258 to X. C.)
the Young Talent Support Plan of Xi’an Jiaotong University (X. C.)
the Technology Foundation for Selected Overseas Chinese Scholar of Shaanxi Province (X. C.)
the Fundamental Research Funds for the Central Universities (2016qngz02to X. C.)
the One Hundred Talents Program of Shaanxi Province (X. C.)
the Natural Science Foundation of Shaanxi Province (2017JM5023 to X. C.)
the open fund of the State Key Laboratory of Military Stomatology (2017KA02 to X. C.)。
