摘要
在防雨棚旱池内以花育25号花生品种为试验材料进行土柱栽培试验,设置正常供水(W0)、轻度干旱胁迫(W1)、中度干旱胁迫(W2)三个水分条件和不施氮肥(N0)、低氮(N1,45 kg·hm^(-2))、中氮(N2,90 kg·hm^(-2))、高氮(N3,135 kg·hm^(-2))四个施氮水平,研究不同土壤水分和氮肥条件对花生叶片生理特性的影响。结果表明,与正常供水条件相比,干旱胁迫降低花生叶面积、净光合速率(Pn)和蒸腾速率(Tr),且降低幅度随干旱胁迫程度的增加而增加。不同水分条件下,适量施用氮肥(N1和N2水平)能提高叶片SOD、POD和CAT等抗氧化酶活性,增强花生对干旱胁迫的适应性。中度干旱胁迫下过量施氮会增加MDA含量,加剧膜脂过氧化程度,抑制生长。施用适量氮肥可提高花生光合作用能力和抗氧化酶活性,对干旱胁迫具有一定的补偿效应。
The drought resistanted peanut variety Huayu25 was used as material for water stress and nitrogen fertilizer experiments and grown in soil columns in the anti-rain canopy.The experiment was designed as 3×4 split plots with soil water as main plot and nitrogen fertilizer as sub-plot.Three levels of soil water condition were:①well-watered condition(W0,80%~85%of field moisture capacity),②slight water stress(W1,60%~65%of field moisture capacity),and③moderate water stress(W2,45%~50%of field moisture capacity).Four levels of nitrogen were:①none nitrogen(N0),②low nitrogen(N1,45 kg·hm^(-2)),③moderate nitrogen(N2,90 kg·hm^(-2)),and④high nitrogen(N3,135 kg·hm^(-2)).The leaf physiological activities of peanut plant were analyzed.The results showed that,compared with well-watered condition,the leaf area,net photosynthetic rate(Pn)and transpiration rate(Tr)of peanut leaves were decreased under drought stress.And the decreasing extent became greater with the increase of drought stress.The appropriate application of nitrogen fertilizer(N1 and N2 levels)could improve the activities of antioxidant enzymes such as SOD,POD and CAT in leaves under different water conditions,and increase the adaptability of peanut to drought stress.Under moderate drought stress,excessive nitrogen application could increase the content of MDA,aggravate the degree of membrane lipid peroxidation,which inhibited the growth of peanut under drought stress.The application of appropriate amount of nitrogen fertilizer could improve the photosynthesis and antioxidant enzyme activities in peanut,indicating that nitrogen had a certain compensatory effect on drought stress.
作者
丁红
成波
张冠初
徐扬
秦斐斐
张智猛
于遒功
DING Hong;CHENG Bo;ZHANG Guan-chu;XU Yang;QIN Fei-fei;ZHANG Zhi-meng;YU Qiu-gong(Shandong Peanut Research Institute,Qingdao 266100,China;Qingdao Academy of Agricultural Sciences,Qingdao 266100,China)
出处
《花生学报》
北大核心
2021年第2期64-68,72,共6页
Journal of Peanut Science
基金
国家自然科学基金(31971854,31971856)
山东省重大科技创新工程(2019JZZY010702)。
关键词
花生
干旱胁迫
氮肥
保护酶活性
peanut
drought stress
nitrogen fertilizer
protective enzyme activity
