摘要
目的:探讨雌二醇对小鼠心肌缺血再灌注损伤急性期的保护能力,并分析其可能机制。方法:采用小鼠心肌急性缺血再灌注模型,利用心脏B超及心肌TTC(2,3,5-氯化三苯基四氮唑)染色研究雌二醇对小鼠心肌缺血再灌注急性期心肌收缩能力及心梗面积的影响作用,Western blot法检测雌二醇对心肌巨噬细胞迁移抑制因子(MIF)及其下游AMP激活的蛋白激酶(AMPK)、乙酰辅酶A羧化酶(ACC)磷酸化水平的作用。结果:雌二醇可改善心肌缺血再灌注损伤后小鼠左室射血分数(EF)及短轴缩短率(FS),差异有统计学意义(P<0.05)。雌二醇作用后缺血再灌注心肌MIF水平升高,AMPK、ACC磷酸化水平升高,差异有统计学意义(P<0.05)。结论:雌二醇能够保护小鼠心肌缺血再灌注损伤急性期的心脏功能,这种保护作用可能通过MIF-AMPK信号通路实现。
Objective:To investigate the protective effect of estradiol on myocardial ischemia-reperfusion injury in mice and its possible mechanism.Methods:B-ultrasonography and myocardial TTC staining were used to study the effect of estradiol on the acute stage of myocardial ischemia-reperfusion model in mice,and protein western blot was used to detect the effect of estradiol on macrophage migration inhibitory factor(MIF)and downstream AMPK and Acetyl CoA carboxylase(ACC)phosphorylation.Results:The left ventricular ejection fraction and short-axis shortening rate of mice after myocardial ischemia-reperfusion injury under estradiol were improved,compared with other groups(P<0.05).MIF level and AMPK and ACC phosphorylation were increased in ischemia-reperfusion myocardium after estradiol treatment(P<0.05).Conclusion:Estradiol may play a protective role in the acute stage of myocardial ischemia-reperfusion injury in mice through the MIF-AMPK signaling pathway.
作者
钱鲁
李卓远
张素勤
QIAN Lu;LI Zhuoyuan;ZHANG Suqin(Department of Cardiology,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China;ECG Room,the Second Affiliated Hospital of Wenzhou Medical University,Wenzhou 325027,China)
出处
《温州医科大学学报》
2021年第7期548-553,共6页
Journal of Wenzhou Medical University
基金
温州市科技计划项目(Y20170275)。
关键词
雌二醇
缺血再灌注损伤
巨噬细胞迁移抑制因子
磷酸腺苷活化蛋白激酶
小鼠
estradiol
ischemia-reperfusion injury
macrophage migration inhibitory factor
adenosine monophosphate activated protein kinase
mice
