摘要
基于课题组前期得到的茄子低温转录组数据,筛选到1个1-氨基环丙烷-1-羧酸(1-aminocyclopropane-1-carboxylic acid,ACC)合成酶基因SmACS。研究发现,SmACS编码的氨基酸序列与大豆ACS的同源性最高,并且含有ACC合成酶特有的7个保守结构域和11个不变的氨基酸残基。该SmACS基因位于第8号染色体上,全长3550 bp,编码序列长1437 bp,含3个内含子和4个外显子,属典型的ACS基因结构。其编码的蛋白质由478个氨基酸组成,分子质量为54.03 kDa,等电点为6.48,亲水系数为-0.206,是一种定位于细胞质的不稳定的非分泌型亲水蛋白;该蛋白的主要构成元件为α-螺旋和无规则卷曲,具有保守的Aminotran_1_2结构域,含有39个磷酸化位点,其中以丝氨酸和苏氨酸为主。SmACS蛋白主要与ACC氧化酶等发生相互作用。该基因启动子中含有脱落酸、乙烯、冷胁迫、水杨酸及伤口响应等有关的顺式作用元件。实时荧光定量聚合酶链式反应(real-time fluorescent quantitative polymerase chain reaction,qRT-PCR)结果证实,SmACS基因受低温、高温、干旱和盐诱导而上调表达,其中尤以低温胁迫诱导效果最为显著。上述结果可为进一步研究茄子SmACS基因的功能奠定理论基础。
Based on the early transcriptome data of eggplant under cold stress,the 1-aminocyclopropane-1-carboxylic acid(ACC)synthase gene SmACS was selected out.It was found that the encoded amino acid sequence of SmACS had the highest homology with soybean ACS protein,and included seven conserved domains unique to ACC synthase and 11 invariant amino acid residues.The SmACS gene was located on the chromosome 8,and the full length of this gene was 3550 bp,whose length of coding sequence(CDS)region was 1437 bp.It contained three introns and four exons,which belonged to a typical ACS gene structure.The SmACS protein consisted of 478 amino acids,with a molecular mass of 54.03 kDa,isoelectric point of 6.48 and hydrophilic index of-0.206.It was an unstable non-secreted hydrophilic protein located in the cytoplasm.The main components of SmACS protein were α-helix and random coils.It had a conserved Aminotran_1_2 domain and contained 39 phosphorylation sites,of which serine and threonine were the main ones.The SmACS protein mainly interacted with ACC oxidase.The promoter of SmACS gene contained Cis-acting elements related to abscisic acid,ethylene,cold stress,salicylic acid and wound responses.The results of real-time fluorescent quantitative polymerase chain reaction(qRT-PCR)confirmed that the expression of SmACS gene was induced by cold,heat,drought and salt stresses,among which cold stress was the most significant.The above results lay theoretical basis for functional identification of SmACS gene from eggplant.
作者
万发香
王连臻
高军
WAN Faxiang;WANG Lianzhen;GAO Jun(School of Life Science and Food Engineering,Huaiyin Institute of Technology,Huai’an 223003,Jiangsu,China)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2021年第3期325-334,共10页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
江苏省淮安市科技计划(HAN201601)。
