摘要
为了评价以乳清分离蛋白为底物制备的乳杆菌A-2静息细胞水解产物的抗氧化活性。采用单因素实验优化乳杆菌A-2静息细胞水解非热处理的乳清蛋白的条件、ORAC(Oxygen Radical Absorption Capacity)法评价不同水解产物的抗氧化活性。结果表明,乳杆菌A-2静息细胞在pH7.0、培养温度在39℃的条件下水解非热处理的乳清蛋白48h,水解产物的抗氧化活性最强,相对ORAC值为0.77mmol TE/L。乳杆菌A-2具有水解乳清蛋白的能力,水解产物具有较强的抗氧化活性,为进一步研制乳酸菌的发酵功能食品提供理论依据。
To investigate antioxidant activity of the resting cell hydrolysate produced by Lactobacillus sp.A-2 using whey protein isolate as the substrate.Single factor experiment was employed to optimize the hydrolysis condition of Lactobacillus sp.A-2 resting cell using non-thermal procession whey protein as substrate.Evaluation of the antioxidant activity of the hydrolysates was carried out by ORAC assay.It showed that non-thermal procession whey protein was hydrolysised at pH7.0 and 39℃for 48h,the antioxidant activity of the hydrolysate was higher,relative ORAC value was 0.77 mmol TE/L.Lactobacillus sp.A-2 has the ability to hydrolyze the whey protein,the hydrolysate possesses higher antioxidant activity,the results provided the theoretical basis for further development of the functional foods fermented by lactic acid bacteria.
作者
藏传刚
吴孝霞
李宏玲
金珊珊
骆训邦
史春兰
曹新
关宏
张杰
ZANG Chuangang;WU Xiaoxia;LI Hongling;JIN Shanshan;LUO Xunbang;SHI Chunlan;CAO Xin;GUAN Hong;ZHANG Jie(Qiqihaer Medical University,Qiqihaer 161006)
出处
《中国食品添加剂》
CAS
北大核心
2021年第6期11-14,共4页
China Food Additives
基金
黑龙江省大学生创新创业训练计划项目(No.20131123002)
齐齐哈尔医学院博士专项科研基金(QY2016B-11)。
关键词
乳清蛋白
乳杆菌
静息细胞
抗氧化活性
whey protein
Lactobacillus sp.
resting cell
antioxidant activity
