摘要
目的探讨慢性阻塞性肺疾病急性加重(AECOPD)患者血清成纤维细胞生长因子7(FGF7)及相关炎性因子的表达。方法采用病例对照研究方法,选择新疆医科大学第一附属医院2016年11月至2020年1月收治的AECOPD患者。根据肺功能检查结果将患者分为轻度组〔第1秒用力呼气容积(FEV1)与用力肺活量(FVC)的比值(FEV1/FVC)<0.70、FEV1占预计值的百分比(FEV1%)≥80%〕、中度组(FEV1/FVC<0.70、50%≤FEV1%<80%)和重度组(FEV1/FVC<0.70、30%≤FEV1%<50%),每组20例;以同期20例肺功能正常的择期行胃肠外、骨科等非胸外科手术患者作为对照。记录患者人口学资料、FEV1/FVC、FEV1%、FVC、用力呼气中期流速占预计值的百分比(MMEF%)、6分钟步行试验(6MWT)及圣·乔治呼吸问卷(SGRQ)评分。采用酶联免疫吸附试验(ELISA)检测血清FGF7、白细胞介素(IL-6、IL-1β)和肿瘤坏死因子-α(TNF-α)水平。采用Pearson相关法分析TNF-α与肺功能的相关性。结果与肺功能正常组相比,轻度、中度和重度组FEV1/FVC、FEV1%、MMEF%和6MWT明显降低,SGRQ评分明显升高,且随病情加重呈持续恶化趋势,重度组与肺功能正常组差异均有统计学意义〔FEV1/FVC:0.39±0.09比0.81±0.04,FEV1%:(38.80±6.28)%比(109.58±13.80)%,MMEF%:(0.34±0.14)%比(2.69±0.99)%,6MWT(m):279.00±41.61比402.85±53.97,SGRQ评分(分):34.95±6.71比2.60±2.06,均P<0.05〕;而且轻度、中度和重度组FGF7水平均较肺功能正常组显著降低(ng/L:6.31±2.65、6.10±1.39、6.64±1.77比8.29±3.51,均P<0.05),但轻度、中度、重度组间差异均无统计学意义(均P>0.05)。与肺功能正常组相比,轻度、中度、重度组IL-6和TNF-α水平均显著升高,其中TNF-α水平随着病情加重呈持续升高趋势,重度组与肺功能正常组比较差异均有统计学意义(ng/L:7.42±2.28比3.83±0.92,P<0.05)。但肺功能正常组与轻度、中度和重度组间IL-1β水平比较差异均无统计学意义。相关性分析显示,TNF-α与F
Objective To investigate the expression of fibroblast growth factor 7(FGF7)and related inflammatory factors in the serum of patients with acute exacerbation of chronic obstructive pulmonary disease(COPD).Methods A case control study was conducted.The patients with AECOPD admitted to the First Affiliated Hospital of Xinjiang Medical University from November 2016 to January 2020 were enrolled.The patients were divided into mild group[forced expiratory volume in one second(FEV1)/forced vital capacity(FVC)ratio(FEV1/FVC)<0.70,FEV1 percentage in predicted value(FEV1%)≥80%],moderate group(FEV1/FVC<0.70,50%≤FEV1%<80%),and severe group(FEV1/FVC<0.70,30%≤FEV1%<50%)based on their lung function test results,with 20 patients in each group,and 20 patients with normal pulmonary function who underwent elective non-thoracic surgery such as gastrointestinal surgery and orthopedics surgery in the same period were selected as controls.The demographic data,FEV1/FVC,FEV1%,FVC,maximum mid-expiratory flow percentage in predicted value(MMEF%),6-minute walking test(6MWT),and St George Respiratory Questionnaire(SGRQ)score were recorded respectively.Serum levels of FGF7,interleukins(IL-6,IL-1β)and tumor necrosis factor-α(TNF-α)were determined by enzyme linked immunosorbent assay(ELISA).Pearson correlation was used to analyze the correlation between TNF-αand lung function.Results Compared with the normal pulmonary function group,the levels of FEV1/FVC,FEV1%,MMEF%and 6MWT in the mild,moderate and severe groups were significantly decreased,and the SGRQ scores were increased,the indicators continued to deteriorate with the aggravation of the disease,the statistical differences were found between severe group and normal pulmonary function group[FEV1/FVC:0.39±0.09 vs.0.81±0.04,FEV1%:(38.80±6.28)%vs.(109.58±13.80)%,MMEF%:(0.34±0.14)%vs.(2.69±0.99)%,6MWT(m):279.00±41.61 vs.402.85±53.97,SGRQ scores:34.95±6.71 vs.2.60±2.06,all P<0.05].Compared with the normal pulmonary function group,the levels of FGF7 in the mild,moderate and se
作者
万秋风
郭志金
才开·莎热丽
魏琴
贾文婷
罗茜
杨婷
史玉娇
谷兴丽
徐思成
Wan Qiufeng;Guo Zhijin;Caikai Shareli;Wei Qin;Jia Wenting;Luo Xi;Yang Ting;Shi Yujiao;Gu Xingli;Xu Sicheng(Department of RICU,Respiratory and Critical Care Medical Center,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomous Region,China;Institute of Clinical Medicine,the First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,Xinjiang Uygur Autonomous Region,China)
出处
《中华危重病急救医学》
CAS
CSCD
北大核心
2021年第4期421-426,共6页
Chinese Critical Care Medicine
基金
新疆维吾尔自治区自然科学基金(2014211C025)。
关键词
慢性阻塞性肺疾病
急性加重
成纤维细胞生长因子7
炎性因子
Chronic obstructive pulmonary disease
Acute exacerbation
Fibroblast growth factor 7
Inflammatory factor
