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格隆溴铵对慢性阻塞性肺疾病大鼠气道炎症和肺损伤的影响 被引量:1

Effects of Glycopyrronium Bromide on airway inflammation and lung injury in rats with chronic obstructive pulmonary disease
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摘要 目的探讨格隆溴铵对慢性阻塞性肺疾病(简称慢阻肺)大鼠气道炎症与肺组织的影响。方法采用随机数字表将SD雄性健康大鼠30只分为对照组、模型组和干预组,每组各10只,模型组与干预组经气管内滴入脂多糖(LPS)联合熏香烟建立慢阻肺大鼠模型,干预组采用格隆溴铵进行干预治疗,观察各组大鼠干预后的气道炎症反应以及肺组织病理变化。结果与对照组相比,模型组、干预组大鼠气管周围、血管周围、肺间质周围炎症情况评分、肿瘤坏死因子-α(tumor necrosis factor-alpha,TNF-α)含量、角质形成细胞衍生趋化因子(keratinocyte-derived chemokine,KC)含量、细胞总数、中性粒细胞百分比、基质金属蛋白酶9(matrix metalloproteinases-9,MMP-9)、基质金属蛋白酶抑制物-1(matrix metalloproteinase inhibitor-1,TIMP-1)表达水平均明显升高(P<0.05),还原型谷胱甘肽(reduced glutathione,GSH)含量、淋巴细胞百分比、单核细胞百分比降低(P<0.05);与模型组相比,干预组大鼠气管周围评分(1.52±0.22)分、血管周围评分(1.20±0.19)分、肺间质周围炎症情况评分(1.10±0.16)分、TNF-α含量(167.33±13.37)pg/mL、KC含量(53.18±6.22)pg/mL、细胞总数(2.52±0.21)106/mL、中性粒细胞百分比(13.02±1.29)%和MMP-9表达水平(1.42±0.06)、TIMP-1表达水平(1.29±0.06)均明显降低(P<0.05),GSH含量(4.97±0.41)μg/mL、淋巴细胞百分比(8.71±0.86)%、单核细胞百分比(78.24±6.94)%均显著升高(P<0.05)。结论格隆溴铵可以降低慢阻肺大鼠肺组织损伤,可能与降低慢阻肺大鼠气道炎症反应有关。 Objective To explore the effects of Glycopyrronium Bromide on airway inflammation and lung injury in rats with chronic obstructive pulmonary disease(COPD).Methods Random number table method was adopted to divide 30 healthy SD male rats into control group,model group and intervention group,with 10 rats in each group.The model group and intervention group were injected with Lipopolysaccharide(LPS)combined with incense smoke to establish COPD rat model.The intervention group was treated with Glycopyrronium Bromide.The airway inflammation and pathological changes of lung tissue of the rats were observed after intervention.Results Compared with the control group,the scores of inflammation around trachea,around blood vessels and around lung interstitium,tumor necrosis factor-alpha(TNF-α)content,keratinocyte-derived chemokine(KC)content,total number of cells,percentage of neutrophils,and the expression levels of matrix metalloproteinases-9(MMP-9)and matrix metalloproteinase inhibitor-1(TIMP-1)in the model group and the intervention group were significantly increased(P<0.05).While reduced glutathione(GSH)content,lymphocyte percentage and monocyte percentage were decreased(P<0.05).Compared to the model group,peritracheal score(1.52±0.22),perivascular score(1.20±0.19),periinterstitial pulmonary inflammation score(1.10±0.16),TNF-αcontent(167.33±13.37)pg/mL,KC content(53.18±6.22)pg/mL,total number of cells(2.52±0.21)106/mL,neutrophil percentage(13.02±1.29)%,expression level of MMP-9(1.42±0.06),and expression level of TIMP-1(1.29±0.06)in the intervention group were significantly decreased(P<0.05).While GSH content(4.97±0.41)μg/mL,lymphocyte percentage(8.71±0.86)%and monocyte percentage(78.24±6.94)%were significantly increased(P<0.05).Conclusion Glycopyrronium Bromide can reduce lung tissue damage in COPD rats,which may be related to the reduction of airway inflammation in COPD rats.
作者 侯云鹤 杨少朋 于超 张学艳 Hou Yunhe;Yang Shaopeng;Yu Chao;Zhang Xueyan
出处 《中国疗养医学》 2021年第8期785-789,共5页 Chinese Journal of Convalescent Medicine
关键词 慢性阻塞性肺疾病 格隆溴铵 气道炎症 基质金属蛋白酶9 Chronic obstructive pulmonary disease Glycopyrronium Bromide Airway inflammation Matrix metalloproteinases-9
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