摘要
为了解四川地区猪源戊型肝炎病毒(hepatitis E virus,HEV)的感染情况,采集四川13个地区屠宰场的358份生猪组织样品,采用荧光RT-PCR进行HEV检测,对阳性样品进行开放阅读框(open reading frame,ORF)两部分序列测序、同源性及系统进化分析。结果显示:猪感染HEV的阳性率为4.75%,17个毒株ORF2部分序列之间同源率为85.4%~99.4%,均属于基因4型,其中4a亚型8株、4b亚型3株、4d亚型6株。与人、猪等不同宿主的HEV参考毒株比较,四川猪源HEV与我国主要流行的人源HEV遗传关系密切,其中10株与人源毒株同源率超过95%。结果表明:四川地区生猪HEV流行毒株以基因4型为主,具有较大的人畜共患风险,应有针对性、系统性地对其进行预防和控制。
To investigate the situation of swine hepatitis E virus(HEV)infection in the Sichuan area,a total of 358 tissue samples were collected from the slaughter houses in the thirteen regions in the area and were tested for HEV nucleic acid using fluorescence RT-PCR.The positive samples were subjected to partial ORF2 sequencing and phylogenetic analysis.The results showed the positive rate of the swine HEV was 4.75%,and the homology between the partial sequences of the open reading frame(ORF)2 among 17 HEV strains were 85.4%-99.4%.All the 17 sequenced strains were clustered into genotype 4,including eight subtype 4 a strains,three subtype 4 b strains and six subtype 4 d strains.Compared with the reference strains from different hosts such as humans and swine,the sequenced HEV strains were closely related to the human HEV strains,the major pandemics in China,with 10 strains having a homology rate higher than 95%.In short,the present study indicated that the HEV strains prevalent in the Sichuan area belonged to genotype 4,which posed a high risk of zoonoses and should be systematically prevented and controlled.
作者
张毅
陈斌
梁璐琪
邵靓
周明忠
裴超信
陈弟诗
丁梦蝶
陈冬
ZHANG Yi;CHEN Bin;LIANG Luqi;SHAO Jing;ZHOU Mingzhong;PEI Chaoxin;CHEN Dishi;DING Mengdie;CHEN Dong(Sichuan Province Center for Animal Disease Prevention and Control,Chengdu 610041,China)
出处
《畜牧与兽医》
北大核心
2021年第6期73-77,共5页
Animal Husbandry & Veterinary Medicine
基金
四川省科技计划应用基础研究项目(2019YJ0559)。
关键词
猪
戊型肝炎病毒
分子流行病学调查
序列分析
基因型
swine
hepatitis E virus
molecular epidemiological investigation
sequence analysis
genotype
