摘要
为探究非洲猪瘟病毒(ASFV)感染猪胃肝淋巴结病理组织学变化特征,并分析不同病毒剂量感染猪的病变差异,通过注射103HAD50(HAD50:半数红细胞吸附量)和104HAD50剂量的ASFV HLJ/18株处理SPF猪后取其胃肝淋巴结,使用HE染色、网状纤维染色、透射电镜等技术观察病理组织学变化,并用统计学软件分析。结果显示:感染ASFV猪胃肝淋巴结正常结构被破坏,被膜下、小梁周围以及皮质区淋巴小结处淤血明显,出血、炎性细胞增多;高剂量感染猪相较低剂量感染猪淋巴结出血更明显,组织正常结构损伤更严重。与正常猪相比,感染猪淋巴结网状纤维变粗,纤维数量显著增加,高剂量感染猪网状纤维数量较低剂量感染猪增多;透射电镜下淋巴结中发现大量ASFV颗粒,主要分布于巨噬细胞和淋巴细胞内,病毒颗粒结构清晰完整,呈典型的正六边形,核呈致密圆形;胃肝淋巴结中巨噬细胞、淋巴细胞的胞核结构被破坏,核皱缩变形,染色质聚集浓缩,线粒体肿胀,甚至出现空泡化;有些细胞发生凋亡。研究结果为进一步阐明ASFV的免疫致病机制和非洲猪瘟(ASF)的防治提供了参考。
In this study, African swine fever virus(ASFV)-infected pigs injected with 103HAD50(HAD50: half of haemadsorption) and 104HAD50 were used as the research subjects to explore the characteristics of histopathological changes in the gastrohepatic lymph nodes, and to analyze the differences in the pathological changes in pigs infected with different virus doses. After the ASFV HLJ/18 strain was used to treat the SPF pigs, the gastrohepatic lymph nodes of the animals were taken. Then, hematoxylin and eosin staining, reticular fiber staining, transmission electron microscope observation and statistical software were used in histopathological analysis of the samples. The results showed that the normal structure of the gastrohepatic lymph nodes in the pigs infected with ASFV was destroyed, and that there was obvious congestion in the subcapsular, trabecular and cortical lymph nodes, with bleeding and increased inflammatory cells. The bleeding of lymph nodes was more obvious and the damage of normal tissue structure was more serious, in the high-dose infected pigs than in the low-dose infected pigs. Compared with the normal pigs, the reticular fibers in the lymph nodes of the infected pigs became thicker and the number of reticular fibers increased significantly(P<0.01). The number of reticular fibers in the high-dose infected pigs was lower than that of the low-dose infected pigs. A large number of ASFV particles were found in the lymph nodes of the infected pigs under transmission electron microscopy, which were mainly distributed in the macrophages and lymphocytes. The structure of the virus particle was clear and complete, in a typical regular hexagonal shape,with a dense round core. The nuclear structure of the macrophages and lymphocytes in the gastrohepatic lymph nodes were destroyed,the nuclei shrank and deformed,and chromatin was aggregated and concentrated. The mitochondria swelled and even blebbed. Some cells underwent apoptosis. These results provided reference for further elucidating the immunopathogenic mech
作者
陈畅
何希君
汪昊
伍靖贤
吴睿智
丁佰韬
步志高
陈秋生
CHEN Chang;HE Xijun;WANG Hao;WU Jingxian;WU Ruizhi;DING Baitao;BU Zhigao;CHEN Qiusheng(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China;Agriculture and Rural Bureau of Binhai County,Yancheng 224523,China)
出处
《畜牧与兽医》
北大核心
2021年第6期41-46,共6页
Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金(31872433)。
