摘要
目的:利用网络药理学方法探讨藏红花抗动脉粥样硬化(atherosclerosis,AS)的主要通路和可能机制。方法:通过TCMSP数据库获得药物有效成分及靶点蛋白,运用Uniprot数据库查询靶点蛋白对应的基因名称,得到藏红花调控的人源靶标。通过DisGeNET数据库及既往文献获取AS的人源靶标。将藏红花的靶点基因与AS靶标基因取交集,获得藏红花治疗AS的靶标基因。借助Cytoscape3.7.2软件构建藏红花活性成分-潜在靶点、藏红花活性成分-AS疾病靶点的相互作用网络,通过网络拓扑特征评价筛选出藏红花在AS疾病中作用的核心靶点;运用Cytoscape3.7.2软件中的ClueGo插件对所得靶点进行GO生物进程及KEGG代谢通路分析。结果:以类药性≥0.18为限定条件及既往文献搜索筛选得到22个化合物,共获得106个藏红花调控的靶点蛋白和117个AS靶标,取交集,获得26个藏红花治疗AS的靶标。在药物-候选成分-AS的候选靶点网络中根据度值得到藏红花抗AS的主要有效成分为藏红花酸、异鼠李素和槲皮素。应用Cytoscape3.7.2软件中的ClueGo插件对26个基因靶标进行KEGG富集分析,获得与藏红花治疗AS相关的通路。运用KEGG数据库并查阅既往文献,获得藏红花抗AS的相关通路示意图。结论:藏红花抗AS可能主要由藏红花中的藏红花酸、异鼠李素及槲皮素等有效化学成分发挥抗炎及抑制血管新生的作用,从而达到抗AS的作用。
Objective:To explore the main pathways and possible mechanisms of saffron in anti-atherosclerosis(AS)us-ing network pharmacology.Methods:The active ingredients and target proteins of the drug were obtained from the TCMSP data-base,and the gene names corresponding to the target proteins were searched using the Uniprot database to obtain human targets regulated by saffron.Human targets of AS were obtained from DisGeNET database and previous literature.The target gene of saf-fron was intersected with the target gene of AS to obtain the target gene of saffron for AS.With the help of Cytoscape3.7.2 soft-ware,a saffron active ingredient-potential target,saffron active ingredient-AS disease target interaction network was constructed,and the core targets of saffron’s role in AS disease were screened out through evaluation of network topological characteristics such as degree;The ClueGo plug-in in Cytoscape3.7.2 software was used to analyze GO biological processes and KEGG metabolic pathways of targets.Results:With the drug-like property≥0.18 as the limiting condition and previous literature search and screening,22 compounds were obtained.A total of 106 saffron-regulated target proteins,and 117 AS targets were obtained.The inter-sections were selected to obtain 26 saffron targets for AS.According to the degree of drug-candidate component-AS candidate tar-get network,the main effective components of saffron anti-AS were crocetin,isorhamnetin and quercetin.A total of 26 gene tar-gets were analyzed by KEGG enrichment using the ClueGo in Cytoscape3.7.2 software to obtain pathways related to saffron treat-ment of AS.Using the KEGG database and consulting previous literature,a schematic diagram of the related pathways of saffron anti-AS was obtained.Conclusion:The anti-AS of saffron may be mainly attributed to crocetin,isorhamnetin and quercetin in saf-fron,which exert anti-inflammatory and inhibit angiogenesis effects,thereby achieving anti-AS effect.
作者
杨静
任星
张菀桐
高蕊
YANG Jing;REN Xing;ZHANG Wan-tong;GAO Rui(Graduate School of Beijing University of Chinese Medcine,Beijing 100029,China;Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)
出处
《海南医学院学报》
CAS
2021年第12期925-931,共7页
Journal of Hainan Medical University
基金
国家重点研发计划“中医药现代化研究”(2019YFC1709300)
国家“重大新药创制”项目(2017ZX09304003)。
