摘要
为了提高细菌纤维素(Bacterial Cellulose,BC)动态发酵产量,从6种腐烂的水果中筛选出产细菌纤维素菌种42株,从所筛菌种中选择初筛动态发酵产量最高的木糖驹形氏杆菌(Komagataeibacter xylinus)P1-1,对P1-1菌株产的细菌纤维素进行性质分析。红外光谱和热重分析结果表明,细菌纤维素有良好的热稳定性,扫描电镜结果表明纤维直径仅有27nm。通过单因素试验及正交试验优化发酵培养基为:葡萄糖60 g/L,安琪蛋白胨FP41014.41 g/L,KH2PO_(4)3 g/L,MgSO_(4)·7H2O20 g/L,乙酸1 mL/L。在此培养基中,木糖驹形氏杆菌P1-1动态发酵96h后细菌纤维素产量达4.30 g/L,比初始培养条件下产量(1.76 g/L)提高了2.44倍。该研究为利用木糖驹形氏杆菌生产细菌纤维素提供了理论依据,并为细菌纤维素进一步动态发酵的研究奠定了基础。
In order to improve the agitated cultureyield of bacterial cellulose(BC),42 strains of bacterial cellulose-producing bacteria were screened from six decayed fruits,and the highest agitated culture yield of Komagataeibacter xylinusP1-1 was selected from the screened strains in the initial screening.The results of FT-IR and TGA analysis showed that the bacterial cellulose has good thermal stability,and the SEM results showed that the fiber diameter was only 27 nm.The fermentation medium was optimized by single-factor test and orthogonal test as follows:glucose 60 g/L,Anchor peptone FP41014.41 g/L,KH2PO_(4)3 g/L,MgSO_(4)·7H2O 20 g/L,and acetic acid 1 mL/L.In this medium,the bacterial cellulose production of K.xylinusP1-1 reached4.30 g/L after 96 h of agitated fermentation,which was 2.44 times higher than that of the initial fermentation medium(1.76 g/L).This study provided a theoretical basis for the production of bacterial cellulose using K.xylinusand laid the foundation for further studies on the agitated culture of bacterialcellulose.
作者
廖博文
黄轶敏
谭玉静
蒋德明
常忠义
邹春静
高红亮
LIAO Bo-wen;HUANG Yi-min;TAN Yu-jing;JIANG De-ming;CHANG Zhong-yi;ZOU Chun-jing;GAO Hong-liang(School of Life Sciences,East China Normal University,Shanghai 200241,China;Shanghai Academy of Quality Supervision and Inspection Technology,Shanghai 200233,China)
出处
《纤维素科学与技术》
CAS
2021年第2期11-21,共11页
Journal of Cellulose Science and Technology
基金
国家自然科学基金项目(31670138)。
