摘要
目的:建立气相色谱法测定糖平煎中苍术酮含量的方法。方法:色谱柱为PEG-20M毛细管色谱柱(30 m×0.25 mm×0.25μm),采用程序升温,进样口温度为290℃,检测器温度为300℃,检测器为氢火焰离子化检测器(flame ionization detector,FID),载气为氮气,流速1 mL·min^(-1),内标物质为萘。结果:被测物苍术酮与内标物质萘分离度均良好,平均回收率为100.1%,RSD为2.64%,糖平煎中苍术酮含量为1.13 mg·g^(-1)。结论:该研究建立的气相色谱方法简便、灵敏度高、数据准确,可用于糖平煎复方中苍术酮含量的测定。
Objective:To establish a gas chromatography method for the determination of atractylone in TangpingJian.Method:PEG-20M capillary column(30 m×0.25 mm×0.25μm)was used as the chromatographic column.The temperature was programmed with the inlet temperature of 290℃and detector temperature of 300℃,and the detector was hydrogen flame ionization detector(FID).The carrier gas was nitrogen with the flow rate of 1 mL·min^(-1),and naphthalene was used as the internal standard substance.Results:The separation of the atractylone and the internal standard substance was good,and the average recovery rate and RSD was 100.1%and 2.64%,respectively.The atractylone content in the Tangpingjian was 1.13 mg·g^(-1).Conclusion:The established gas chromatographic method is simple,highly sensitive and accurate.It can be used for the detection of atractylone content in TangpingJian.
作者
田嘉铭
于小红
王银月
王旭
王治宝
王金
卢金铭
TIAN Jia-ming;YU Xiao-hong;WANG Yin-yue;WANG Xu;WANG Zhi-bao;WANG Jin;LU Jin-ming(Department of Pharmacy,Hebei North University,Zhangjiakou,075000,China;Hebei Provincial Key Laboratory of Neuropharmacology,Zhangjiakou,075000,China;Hebei Building Engineering School,Baoding,071000,China)
出处
《神经药理学报》
2020年第5期14-17,共4页
Acta Neuropharmacologica
基金
张家口市科技局项目(No.1411063C)。
关键词
糖平煎
苍术酮
含量测定
气相色谱法
tangpingjian
atractylone
determination
gas chromatography
