摘要
目的:探究载脂蛋白M(ApoM)缺失对肾小管上皮细胞(RTECs)增殖及凋亡的影响,为肾脏疾病的防治提供新的思路。方法:收集野生型小鼠(WT)及ApoM基因缺失型(ApoM^(-/-))小鼠尿液并检测尿蛋白、尿酸及尿肌酐的含量。原代培养两组小鼠的RTECs,EdU试剂盒检测其增殖活力;Western blot法检测凋亡相关关键蛋白。结果:ApoM^(-/-)小鼠尿蛋白和尿酸增多,且尿蛋白和尿肌酐比值增高;ApoM^(-/-)小鼠的RTECs增殖活力下降且凋亡水平上调。结论:ApoM基因的缺失造成小鼠肾功能受损,且抑制了RTECs的增殖并上调其凋亡水平。
Objective:To investigate the effect of apolipoprotein M(ApoM)deletion on the proliferation and apoptosis of renal tubular epithelial cells(RTECs)for new route in prevention of treatment of renal disorders.Methods:Urine samples were collected from mice of wild-type(WT)and ApoM deletion(ApoM^(-/-)),and measured for the content of urine protein,uric acid and urine creatinine.Then RTECs obtained from the two groups of mice were subjected to primary culture,and observed for their proliferation activity using commercial EdU kit.Western blot was performed to detect the key protein associated with the apoptosis of RTECs.Results:The level of urine protein and uric acid as well as the ratio of urine protein and creatinine was increased in ApoM^(-/-)mice.Also,decreased proliferation activity of RTECs,yet increased apoptosis was seen in ApoM^(-/-)mice.Conclusion:ApoM gene deletion can lead to impaired renal function in mice,and inhibit the proliferation of RTECs via up-regulating apoptosis.
作者
白亚萍
裴文俊
吴亚丽
闵娇
花长春
刘洁
章尧
BAI Yaping;PEI Wenjun;WU Yali;MIN Jiao;HUA Changchun;LIU Jie;ZHANG Yao(Anhui Key Laboratory of Biomacromolecule Research,Wannan Medical College,Wuhu 241002,China)
出处
《皖南医学院学报》
CAS
2021年第3期210-212,216,共4页
Journal of Wannan Medical College
基金
安徽省重点研究和开发计划(1804h0802024)
安徽高校自然科学研究项目(KL2020A0612)。
关键词
肾小管上皮细胞
载脂蛋白M
增殖
凋亡
renal tubular epithelial cells
apolipoprotein M
proliferation
apoptosis
