摘要
利用沸石良好的吸氨能力和微生物载体功能,将受饥饿影响的厌氧氨氧化菌在含沸石的反应器内进行活性恢复,研究沸石对长期饥饿影响下厌氧氨氧化菌恢复过程中菌群活性及结构的影响。结果表明,经过82天的运行,沸石反应器和对照反应器(无沸石添加)内厌氧氨氧化菌均获得恢复。沸石反应器的脱氮效率从第59天开始与对照反应器拉开差距,运行第82天时,沸石反应器中氮去除负荷(NRR)为177.8 mgN/(L·d),显著大于对照反应器(154.3 mgN/(L·d))。利用16S rRNA高通量测序方法,对反应器内菌群的微生物组成与功能进行分析,发现投加沸石载体使得恢复后的厌氧氨氧化菌Candidatus Brocadia的丰度更高,可以更好地促进受饥饿抑制的厌氧氨氧化菌的恢复。
Zeolite has good ammonium absorption capacity and microbial carrier function.Anammox bacteria affected by starvation were activated in a reactor containing zeolite to study the effect of zeolite on the activity and structure of anammox bacteria after long-term starvation.The results showed that anammox bacteria recovered in the zeolite reactor and the control reactor(without zeolite addition)after 82 days of operation.The nitrogen removal efficiency of zeolite reactor began to lag behind that of the control reactor on the 59th day.On the 82nd day of operation,the nitrogen removal load(NRR)of zeolite reactor was 177.8 mgN/(L·d),significantly higher than that of the control reactor(154.3 mgN/(L·d)).At the same time,16S rRNA high-throughput sequencing method was used to analyze the microbial composition and function of the microbial community in the reactor.It was found that adding zeolite carrier could increase the abundance of anammox bacteria Candidatus brocadia after recovery,and could better promote the recovery of anammox bacteria inhibited by hunger.
作者
余道道
孙敬起
霍唐燃
赵云鹏
刘思彤
YU Daodao;SUN Jingqi;HUO Tangran;ZHAO Yunpeng;LIU Sitong(Department of Environment and Energy,Peking University Shenzhen Graduate School,Shenzhen 518055;Department of Environmental Science&Engineering,Peking University,Beijing 100871)
出处
《北京大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2021年第3期507-516,共10页
Acta Scientiarum Naturalium Universitatis Pekinensis
关键词
厌氧氨氧化菌
沸石
细菌恢复
群落结构
anammox bacteria
zeolite
bacterial recovery
community structure
